不同血清对马脂肪源间充质干细胞(hrs-AT MSC)培养和生长模式的影响

Subodh Kumar Maiti, G. Wouters, D. Spitkovsky, J. Hescheler
{"title":"不同血清对马脂肪源间充质干细胞(hrs-AT MSC)培养和生长模式的影响","authors":"Subodh Kumar Maiti, G. Wouters, D. Spitkovsky, J. Hescheler","doi":"10.15406/jsrt.2021.07.00150","DOIUrl":null,"url":null,"abstract":"Equine multipotent mesenchymal stem cells (hrs-MSC) can be isolated from various tissues including adipose tissue (AT). We have analyzed the effect of different serum sources on hrs-AT-MSC cultured and assessed proliferation, morphology, viability and immunophenotype and plasticity. The hrs-AT-MSC was cultured separately with growth media containing three different serums: 20% FCS (Gibco), 20% horse serum (Sigma) and 20% allogenic horse serum (Belgium lab) in CO2 incubators. The hrs-AT-MSC growth and proliferation was better in cultural conditions where 20% FCS and 20% horse serum (Sigma) were used. Mesenchymal stem cell count was highest in the condition where horse serum (sigma) was used than both FCS and horse allogenic serum. The viability was more in where allogenic serum (Belgium lab) was used than both FCS (Gibco) and horse serum (Sigma). Like FCS (Gibco), horse serum (Sigma) and allogenic horse serum (Belgium lab) also showed promising /positive effects on equine adipose tissue derived mesenchymal stem cell (hrs-At-MSC) culture and proliferation. Horse serum was found as efficient as fetal calf serum in supporting proliferation and differentiation of equine mesenchymal stem cells in vitro. Further studies are needed to analyze these aspects of MSC in tissue regeneration. Stem cell biology has attracted tremendous interest recently. It is hoped that it will play a major role in the treatment of a number of incurable diseases via transplantation therapy. Several verities of stem cells have been isolated and identified in vivo and in vitro. Very broadly they comprise of two major classes: embryonic and adult mesenchymal stem cells.1 Mesenchymal stem cells (MSCs) because of their self replication, differentiate into various types of mature cells and tissues, and regeneration capabilities are regarded as an excellent source of cells for tissue engineering and for treatment of various incurable diseases and therapeutic uses in gene therapy, drug delivery, and reconstructive surgery.2,3 Recently, induced pluripotent stem cells (iPSC) and embryonic stem cells (ESCs) attracted researchers in organogenesis and cell-mediated therapy experiments, however, teratoma formation, ethical issues, and graft vs host rejection are the major limitations in development and therapeutic application of these cells.4 Due to these limitations, mesenchymal stem cells (MSCs) from adult tissues are now attractive material for and tissue engineering and cell-mediated therapy.5 Isolation of MSC derived from equine species has been reported in a number of different tissues, including bone marrow,6 peripheral blood,7 fat tissue8 and umbilical cord blood.9 Adipose tissue derived equine MSC (hrs-AT-MSC) exhibit the ability to differentiate into different types of cells and tissues in appropriate culture conditions using growth factors and specific hormones into osteoblast, chondroblat and adipocytes and a profound proliferative ability without hampering their own genetic firmness.8 Serum is an integral component for MSC culture in vitro and also it is essential for osteogenic differentiation of MSC along with other factors includes β glycerophosphate, ascorbic acid, and dexamethasone as osteogenic supplements. 10 Serum is an essential component of complete growth media for MSC culture because it provides growth factors, nutrients and extracellular matrix proteins which support MSC cells in vitro.11,12 There is also evidence that serum may act as an antioxidant for cells.13 Despite its zoogenic content, animal serum has been used since the first isolation of MSCs and remains a prime component for their culture and differentiation. Different methods for reducing animal antigens in fetal calf serum (FCS) have been suggested but none alleviate 100% percent risks,14 which leads to current researches for the development of substitute culture conditions, and a move towards the possible use of cheap, readily available as well as potentiate serum from other sources. The purpose of this study was to use three different types of serum for the culture and proliferation of equine adipose tissue derived mesenchymal stem cells (hrs-AT-MSC).","PeriodicalId":172569,"journal":{"name":"Journal of Stem Cell Research & Therapeutics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effect of different serums on culture and growth pattern on equine adipose derived mesenchymal stem cells (hrs-AT MSC)\",\"authors\":\"Subodh Kumar Maiti, G. Wouters, D. Spitkovsky, J. Hescheler\",\"doi\":\"10.15406/jsrt.2021.07.00150\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Equine multipotent mesenchymal stem cells (hrs-MSC) can be isolated from various tissues including adipose tissue (AT). We have analyzed the effect of different serum sources on hrs-AT-MSC cultured and assessed proliferation, morphology, viability and immunophenotype and plasticity. The hrs-AT-MSC was cultured separately with growth media containing three different serums: 20% FCS (Gibco), 20% horse serum (Sigma) and 20% allogenic horse serum (Belgium lab) in CO2 incubators. The hrs-AT-MSC growth and proliferation was better in cultural conditions where 20% FCS and 20% horse serum (Sigma) were used. Mesenchymal stem cell count was highest in the condition where horse serum (sigma) was used than both FCS and horse allogenic serum. The viability was more in where allogenic serum (Belgium lab) was used than both FCS (Gibco) and horse serum (Sigma). Like FCS (Gibco), horse serum (Sigma) and allogenic horse serum (Belgium lab) also showed promising /positive effects on equine adipose tissue derived mesenchymal stem cell (hrs-At-MSC) culture and proliferation. Horse serum was found as efficient as fetal calf serum in supporting proliferation and differentiation of equine mesenchymal stem cells in vitro. Further studies are needed to analyze these aspects of MSC in tissue regeneration. Stem cell biology has attracted tremendous interest recently. It is hoped that it will play a major role in the treatment of a number of incurable diseases via transplantation therapy. Several verities of stem cells have been isolated and identified in vivo and in vitro. Very broadly they comprise of two major classes: embryonic and adult mesenchymal stem cells.1 Mesenchymal stem cells (MSCs) because of their self replication, differentiate into various types of mature cells and tissues, and regeneration capabilities are regarded as an excellent source of cells for tissue engineering and for treatment of various incurable diseases and therapeutic uses in gene therapy, drug delivery, and reconstructive surgery.2,3 Recently, induced pluripotent stem cells (iPSC) and embryonic stem cells (ESCs) attracted researchers in organogenesis and cell-mediated therapy experiments, however, teratoma formation, ethical issues, and graft vs host rejection are the major limitations in development and therapeutic application of these cells.4 Due to these limitations, mesenchymal stem cells (MSCs) from adult tissues are now attractive material for and tissue engineering and cell-mediated therapy.5 Isolation of MSC derived from equine species has been reported in a number of different tissues, including bone marrow,6 peripheral blood,7 fat tissue8 and umbilical cord blood.9 Adipose tissue derived equine MSC (hrs-AT-MSC) exhibit the ability to differentiate into different types of cells and tissues in appropriate culture conditions using growth factors and specific hormones into osteoblast, chondroblat and adipocytes and a profound proliferative ability without hampering their own genetic firmness.8 Serum is an integral component for MSC culture in vitro and also it is essential for osteogenic differentiation of MSC along with other factors includes β glycerophosphate, ascorbic acid, and dexamethasone as osteogenic supplements. 10 Serum is an essential component of complete growth media for MSC culture because it provides growth factors, nutrients and extracellular matrix proteins which support MSC cells in vitro.11,12 There is also evidence that serum may act as an antioxidant for cells.13 Despite its zoogenic content, animal serum has been used since the first isolation of MSCs and remains a prime component for their culture and differentiation. Different methods for reducing animal antigens in fetal calf serum (FCS) have been suggested but none alleviate 100% percent risks,14 which leads to current researches for the development of substitute culture conditions, and a move towards the possible use of cheap, readily available as well as potentiate serum from other sources. The purpose of this study was to use three different types of serum for the culture and proliferation of equine adipose tissue derived mesenchymal stem cells (hrs-AT-MSC).\",\"PeriodicalId\":172569,\"journal\":{\"name\":\"Journal of Stem Cell Research & Therapeutics\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-03-18\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Stem Cell Research & Therapeutics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15406/jsrt.2021.07.00150\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stem Cell Research & Therapeutics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15406/jsrt.2021.07.00150","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

马多能间充质干细胞(hrs-MSC)可以从多种组织中分离出来,包括脂肪组织(AT)。我们分析了不同血清来源对hrs-AT-MSC培养的影响,并评估了其增殖、形态、活力、免疫表型和可塑性。将hrs-AT-MSC分别用含有三种不同血清(20% FCS (Gibco)、20%马血清(Sigma)和20%同种异体马血清(比利时实验室))的生长培养基在CO2培养箱中培养。在20% FCS和20%马血清(Sigma)的培养条件下,hrs-AT-MSC的生长和增殖效果较好。马血清(sigma)组间充质干细胞计数高于FCS和马同种异体血清组。同种异体血清(比利时实验室)的存活率高于FCS (Gibco)和马血清(Sigma)。与FCS (Gibco)一样,马血清(Sigma)和同种异体马血清(比利时实验室)对马脂肪组织源性间充质干细胞(hhs - at - msc)的培养和增殖也显示出良好/积极的影响。马血清与胎牛血清在体外支持马间充质干细胞的增殖和分化方面具有相同的效果。需要进一步的研究来分析MSC在组织再生中的这些方面。干细胞生物学近年来引起了人们极大的兴趣。希望它将在通过移植治疗许多无法治愈的疾病中发挥重要作用。已经在体内和体外分离和鉴定了几种真实的干细胞。大体上,它们包括两大类:胚胎间充质干细胞和成体间充质干细胞间充质干细胞(MSCs)由于其自我复制能力,可分化成各种类型的成熟细胞和组织,其再生能力被认为是组织工程、治疗各种不治之症以及基因治疗、药物输送和重建手术等治疗用途的良好细胞来源。近年来,诱导多能干细胞(iPSC)和胚胎干细胞(ESCs)在器官发生和细胞介导的治疗实验中备受关注,但畸胎瘤的形成、伦理问题和移植物对宿主的排斥反应是这些细胞发展和治疗应用的主要限制因素由于这些限制,来自成人组织的间充质干细胞(MSCs)现在是组织工程和细胞介导治疗的有吸引力的材料从马身上分离出的间充质干细胞已被报道在许多不同的组织中,包括骨髓、外周血、脂肪组织和脐带血脂肪组织来源的马间充质干细胞(hrs-AT-MSC)表现出在适当的培养条件下,利用生长因子和特定激素分化成成骨细胞、软骨细胞和脂肪细胞的不同类型的细胞和组织的能力,以及深刻的增殖能力,而不妨碍其自身的遗传稳定性血清是间充质干细胞体外培养不可或缺的组成部分,也是间充质干细胞成骨分化所必需的,其他因素包括β甘油磷酸酯、抗坏血酸和地塞米松作为成骨补充剂。血清是MSC培养完全生长培养基的重要组成部分,因为它提供生长因子、营养物质和细胞外基质蛋白,在体外支持MSC细胞。也有证据表明血清可以作为细胞的抗氧化剂尽管含有动物源性成分,但自首次分离MSCs以来,动物血清一直被使用,并且仍然是其培养和分化的主要成分。已经提出了减少胎牛血清(FCS)中动物抗原的不同方法,但没有一种方法能100%降低风险,14这导致了目前对替代培养条件的开发研究,并朝着可能使用廉价、易得以及来自其他来源的增强血清的方向发展。本研究的目的是使用三种不同类型的血清培养和增殖马脂肪组织来源的间充质干细胞(hrs-AT-MSC)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of different serums on culture and growth pattern on equine adipose derived mesenchymal stem cells (hrs-AT MSC)
Equine multipotent mesenchymal stem cells (hrs-MSC) can be isolated from various tissues including adipose tissue (AT). We have analyzed the effect of different serum sources on hrs-AT-MSC cultured and assessed proliferation, morphology, viability and immunophenotype and plasticity. The hrs-AT-MSC was cultured separately with growth media containing three different serums: 20% FCS (Gibco), 20% horse serum (Sigma) and 20% allogenic horse serum (Belgium lab) in CO2 incubators. The hrs-AT-MSC growth and proliferation was better in cultural conditions where 20% FCS and 20% horse serum (Sigma) were used. Mesenchymal stem cell count was highest in the condition where horse serum (sigma) was used than both FCS and horse allogenic serum. The viability was more in where allogenic serum (Belgium lab) was used than both FCS (Gibco) and horse serum (Sigma). Like FCS (Gibco), horse serum (Sigma) and allogenic horse serum (Belgium lab) also showed promising /positive effects on equine adipose tissue derived mesenchymal stem cell (hrs-At-MSC) culture and proliferation. Horse serum was found as efficient as fetal calf serum in supporting proliferation and differentiation of equine mesenchymal stem cells in vitro. Further studies are needed to analyze these aspects of MSC in tissue regeneration. Stem cell biology has attracted tremendous interest recently. It is hoped that it will play a major role in the treatment of a number of incurable diseases via transplantation therapy. Several verities of stem cells have been isolated and identified in vivo and in vitro. Very broadly they comprise of two major classes: embryonic and adult mesenchymal stem cells.1 Mesenchymal stem cells (MSCs) because of their self replication, differentiate into various types of mature cells and tissues, and regeneration capabilities are regarded as an excellent source of cells for tissue engineering and for treatment of various incurable diseases and therapeutic uses in gene therapy, drug delivery, and reconstructive surgery.2,3 Recently, induced pluripotent stem cells (iPSC) and embryonic stem cells (ESCs) attracted researchers in organogenesis and cell-mediated therapy experiments, however, teratoma formation, ethical issues, and graft vs host rejection are the major limitations in development and therapeutic application of these cells.4 Due to these limitations, mesenchymal stem cells (MSCs) from adult tissues are now attractive material for and tissue engineering and cell-mediated therapy.5 Isolation of MSC derived from equine species has been reported in a number of different tissues, including bone marrow,6 peripheral blood,7 fat tissue8 and umbilical cord blood.9 Adipose tissue derived equine MSC (hrs-AT-MSC) exhibit the ability to differentiate into different types of cells and tissues in appropriate culture conditions using growth factors and specific hormones into osteoblast, chondroblat and adipocytes and a profound proliferative ability without hampering their own genetic firmness.8 Serum is an integral component for MSC culture in vitro and also it is essential for osteogenic differentiation of MSC along with other factors includes β glycerophosphate, ascorbic acid, and dexamethasone as osteogenic supplements. 10 Serum is an essential component of complete growth media for MSC culture because it provides growth factors, nutrients and extracellular matrix proteins which support MSC cells in vitro.11,12 There is also evidence that serum may act as an antioxidant for cells.13 Despite its zoogenic content, animal serum has been used since the first isolation of MSCs and remains a prime component for their culture and differentiation. Different methods for reducing animal antigens in fetal calf serum (FCS) have been suggested but none alleviate 100% percent risks,14 which leads to current researches for the development of substitute culture conditions, and a move towards the possible use of cheap, readily available as well as potentiate serum from other sources. The purpose of this study was to use three different types of serum for the culture and proliferation of equine adipose tissue derived mesenchymal stem cells (hrs-AT-MSC).
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信