尼日利亚纳萨拉瓦州拉菲亚市出售的一些蔬菜的细菌分析。

Emmanuel Oboh, P. Oleghe
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引用次数: 0

摘要

拉菲亚现代市场销售的新鲜菠菜和卷心菜在拉菲亚Nasarawa州立理工学院微生物实验室进行了细菌学分析,取1g样品在10ml蛋白水中均质。将10倍连续稀释后的等温物用倾板法接种于不同的细菌培养基中,37℃,接种24小时。使用标准程序对菌落总数进行计数和鉴定。结果表明,菠菜在营养琼脂、麦康基琼脂和沙门氏菌-志贺菌琼脂上的平均细菌数最高,分别为1.4 × 104cfu /g、9.2 × 103cfu /g和6.1 × 103cfu /g,白菜在营养琼脂、麦康基琼脂和沙门氏菌-志贺菌琼脂上的平均细菌数分别为1.0 × 104cfu /g、6.0 × 103cfu /g和4.1 × 103CFU/g。分离出的细菌属分别为链球菌(8%)、枯草芽孢杆菌和铜绿假单胞菌(各占15%)、金黄色葡萄球菌(16%)、大肠杆菌和沙门氏菌(各占23%)。这些分离的细菌对公共卫生具有重要意义,因为它们与食源性疾病有关。建议提高整个蔬菜加工价值链的卫生水平,以防止或减少细菌污染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BACTERIOLOGICAL-PROFILE OF SOME VEGETABLES SOLD IN LAFIA METROPOLIS, NASARAWA STATE, NIGERIA.
The bacteriological profile of fresh spinach and cabbage sold in Lafia Modern Market were analyzed in the Microbiology laboratory of Nasarawa State Polytechnic, Lafia by homogenizing 1g of the sample in 10 ml of peptone water. An aliquot from a 10-fold serial dilution was inoculated using the pour plate method into the different bacteriological medium at 37oC for 24 hours. The total number of colonies was counted and identified using standard procedures. Results indicated that spinach had the highest average bacteria count of 1.4 x104CFU/g, 9.2x103CFU/g, and 6.1x103CFU/g Nutrient agar, MacConkey agar, and Salmonella-Shigella agar respectively, while cabbage had an average bacterial count of 1.0x104CFU/g, 6.0x103CFU/g and 4.1x103 CFU/g on Nutrient agar, MacConkey agar, and Salmonella-Shigella agar respectively. The genera of the bacteria isolates identified were Streptococcus spp (8%), Bacillus subtilis and Pseudomonas aeruginosa (15% each), Staphylococcus aureus (16%), Escherichia coli and Salmonella spp (23% each). These isolated bacteria are of public health importance due to their implication in food-borne illnesses. It is recommended that the hygiene level of the entire vegetable processing value chain should be improved in other to prevent or reduce bacterial contamination.
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