{"title":"实验方法","authors":"A. Fahlgren","doi":"10.2307/j.ctt7zth8g.5","DOIUrl":null,"url":null,"abstract":"Materials and reagents Tissue culture reagents, including minimum essential medium Eagle (MEME), RPMI1640 medium, penicillin, streptomycin, newborn calf serum (NCS) and fetal bovine serum (FBS); chitosan (low molecular weight, 50−190 kDa, 75% deacetylation), Histopaque-1077, collagenase (type XI), poly-L-lysine, fluorescein isothiocyanate (FITC) and streptozotocin were from Sigma-Aldrich (Poole, UK). Heparin sodium salt was provided by AppliChem (Darmstadt, Germany). Hanks’ buffered salt solution (HBSS) was from PAA Laboratories (Pasching, Austria). Rabbit polyclonal antibody to insulin was purchased from Gene Tex (Irvine, CA, USA). Goat anti-rabbit IgG (H+L)-HRP conjugate was provided by Alpha Diagnostic International (San Antonio, TX, USA). Dako Liquid DAB + substrate chromogen system was purchased from Dako (Glostrup, Denmark).","PeriodicalId":341856,"journal":{"name":"Composition Analysis of Writing Materials in Cairo Genizah Documents","volume":"130 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"72","resultStr":"{\"title\":\"Experimental Methods\",\"authors\":\"A. Fahlgren\",\"doi\":\"10.2307/j.ctt7zth8g.5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Materials and reagents Tissue culture reagents, including minimum essential medium Eagle (MEME), RPMI1640 medium, penicillin, streptomycin, newborn calf serum (NCS) and fetal bovine serum (FBS); chitosan (low molecular weight, 50−190 kDa, 75% deacetylation), Histopaque-1077, collagenase (type XI), poly-L-lysine, fluorescein isothiocyanate (FITC) and streptozotocin were from Sigma-Aldrich (Poole, UK). Heparin sodium salt was provided by AppliChem (Darmstadt, Germany). Hanks’ buffered salt solution (HBSS) was from PAA Laboratories (Pasching, Austria). Rabbit polyclonal antibody to insulin was purchased from Gene Tex (Irvine, CA, USA). Goat anti-rabbit IgG (H+L)-HRP conjugate was provided by Alpha Diagnostic International (San Antonio, TX, USA). Dako Liquid DAB + substrate chromogen system was purchased from Dako (Glostrup, Denmark).\",\"PeriodicalId\":341856,\"journal\":{\"name\":\"Composition Analysis of Writing Materials in Cairo Genizah Documents\",\"volume\":\"130 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-10-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"72\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Composition Analysis of Writing Materials in Cairo Genizah Documents\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2307/j.ctt7zth8g.5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Composition Analysis of Writing Materials in Cairo Genizah Documents","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2307/j.ctt7zth8g.5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 72
摘要
组织培养试剂，包括最低必需培养基Eagle (MEME)、RPMI1640培养基、青霉素、链霉素、新生牛犊血清(NCS)、胎牛血清(FBS);肝素钠盐由AppliChem (Darmstadt, Germany)提供。Hanks的缓冲盐溶液(HBSS)来自PAA Laboratories (Pasching, Austria)。兔胰岛素多克隆抗体购自Gene Tex (Irvine, CA, USA)。山羊抗兔IgG (H+L)-HRP偶联物由Alpha Diagnostic International (San Antonio, TX, USA)提供。Dako Liquid DAB +底物显色系统购自Dako (Glostrup, Denmark)。
Materials and reagents Tissue culture reagents, including minimum essential medium Eagle (MEME), RPMI1640 medium, penicillin, streptomycin, newborn calf serum (NCS) and fetal bovine serum (FBS); chitosan (low molecular weight, 50−190 kDa, 75% deacetylation), Histopaque-1077, collagenase (type XI), poly-L-lysine, fluorescein isothiocyanate (FITC) and streptozotocin were from Sigma-Aldrich (Poole, UK). Heparin sodium salt was provided by AppliChem (Darmstadt, Germany). Hanks’ buffered salt solution (HBSS) was from PAA Laboratories (Pasching, Austria). Rabbit polyclonal antibody to insulin was purchased from Gene Tex (Irvine, CA, USA). Goat anti-rabbit IgG (H+L)-HRP conjugate was provided by Alpha Diagnostic International (San Antonio, TX, USA). Dako Liquid DAB + substrate chromogen system was purchased from Dako (Glostrup, Denmark).
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