乙醇浓度对脉冲电场杀菌大肠杆菌的影响

Yuichi Murakami, Y. Muramoto
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引用次数: 0

摘要

本研究利用脉冲电场(PEF)检测了乙醇溶液的杀伤效果。我们将PEF(场强:5.5 kV/mm,脉冲长度:4 $\mu$s,施加PEF次数:10-1,000,频率:10 Hz)应用于含有大肠杆菌的0%-40%乙醇溶液的液体样品。研究结果如下:(i)大肠杆菌的存活计数随着施加脉冲次数的增加而减少。(ii)随着乙醇浓度和样品温度的升高,大肠杆菌存活计数降低;脉冲应用后,PEF在30%-40%乙醇溶液中杀死大肠杆菌。(iii)随着样品温度的升高,大肠杆菌的存活计数降低。对于高温下的20%乙醇样品,PEF实验后我们无法观察到大肠杆菌的菌落。(iv)室温样品中PEF灭菌(100脉冲应用)的能耗小于清酒巴氏灭菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Ethanol Concentration on Escherichia coli Sterilization using Pulsed Electric Field Application
This study examined the killing effects of ethanol solution using a pulsed electric field (PEF). We applied PEF (field intensity: 5.5 kV/mm, pulse length: 4 $\mu$s, number of applied PEF: 10-1,000, frequency: 10 Hz) to a liquid sample that is 0%–40% ethanol solution, which contains Escherichia coli. The results are as follows; (i) The survival counts of E. coli decreased with an increase in the number of applied pulses. (ii) The survival counts of E. coli decreased as ethanol concentrations and sample temperatures increased; PEF kills E. coli in 30%–40% ethanol solution after pulse application. (iii) The survival counts of E. coli decreased with an increase in the sample temperature. For the 20% ethanol sample at high temperature, we cannot observe the colonies of E. coli after PEF experiments. (iv) The energy consumption for the PEF sterilization (100 pulses application) in a room temperature sample is less than that for sake pasteurization.
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