Ilg Nurtjahjaningsih, Purnamila Sulistyawati, Anto Rimbawanto
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摘要

本研究旨在利用SSR (simple sequences repeats,简称SSR)标记对一种源种林Calophyllum inophyllum的遗传结构进行评价,以控制其遗传多样性。采集叶片样本,构建DNA模板,使用6条SSR引物。用FSTAT软件分析280株树的遗传数据,用GeneAlex软件分析PCoA。结果表明,每个引物的等位基因数(N A)在3 ~ 4之间,等位基因丰富度(A R)在2.805 ~ 4.000之间,基因多样性(H E)在0.018 ~ 0.631之间,除NY3外,其余引物的近交系数(F IS)均不显著偏离Hardy-Weinberg平衡。TBP的平均H - E值较低(平均H - E = 0.272)。而近交系数均值s不显著。PCoA分析表明,该种子林由遗传相关的树木组成;这280棵树起源于5-6棵母树。这是因为该林分起源于遗传分化较窄的保护林分。对种子林的管理进行了探讨。
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STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS
This study aimed to assess genetic structure of Calophyllum inophyllum using simple sequences repeats (SSR) markers to control genetic diversity at a provenance seed stand. Leaf samples were collected for DNA templates and 6 SSR primers were used. Genetic data of 280 trees were analyzed by FSTAT software , while PCoA was analyzed by GeneAlex. Results showed that number of allele (N A ) per SSR primer between 3 and 4, the allelic richness (A R ) ranged 2.805 to 4.000, the gene diversity (H E ) ranged 0.018 to 0.631 , coefficient inbreeding (F IS ) of overall SSR primers were insignificant deviate from Hardy-Weinberg Equilibrium, excepted NY3. The mean H E values in the TBP was in low (mean H E = 0.272). However, the mean coefficient inbreeding value s was not significant. PCoA analysis showed that the seed stand consisted genetically related trees; the 280 trees originated from only 5-6 mother trees. This is because the stand was originated from a conservation stand with narrow genetic di fferentiation . Management of the seed stand was discussed.
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