非洲莴苣叶提取物合成纳米镍的表征及抗菌性能

A. A. Adekanmi, Owolabi Oluwafemi Akinkunmi, Ifeoluwa, David Ajewole, Lawal, Ibraheem Kehinde, Oyero, Gbenga Micheal, Akinlade Ridwan Young
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引用次数: 0

摘要

作为以前使用的昂贵和危险化学品的替代品,镍纳米颗粒(NiNPs)是利用当地可获得的生物多样性植物制造的。本研究的目的是研究从非洲生菜或野生生菜中提取的镍纳米颗粒的抗菌能力。在尼日利亚奥孙州的埃萨奥克,人们收集了非洲莴苣(lauaea taraxacifolia)的叶子。早上,使用无菌容器收集尿液。尿液样本用C.L.E.D.培养基、MacConkey琼脂和Sabouraud葡萄糖琼脂进行评估和培养。采用形态学、革兰氏染色、生化分析和糖发酵等传统方法对菌株进行鉴定。非洲莴苣在蒸馏水中洗净后切成小片。采用传统方法从非洲生菜叶提取物中制备镍纳米颗粒。采用滴涂法(SEM)对纳米颗粒进行了表征。根据Bergy的细菌鉴定手册,分离物被鉴定为克雷伯氏菌和微球菌。采用琼脂孔扩散法对镍纳米颗粒和非洲生菜叶提取物对克雷伯氏菌和微球菌的抑菌活性进行了研究。综合透射电子显微镜(TEM)照片清楚地表明,镍纳米颗粒具有相同的形态和尺寸,根据研究结果产生的纳米颗粒。TEM图像中可见直径分布在10-20纳米的镍纳米颗粒。纳米镍对克雷伯氏菌和微球菌的抑制区分别为2 mm和0 mm,而对牛蒡提取物的抑制区则为5 mm和7 mm。与制备的镍纳米颗粒(微球菌sp和克雷比氏菌sp)的抑制区相比,植物提取物(龙爪草)对细菌的抑制效果较好。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization And Antimicrobial Property Of Nickel Nanoparticle Synthesized Using Leaves Extract Of Launaea Taraxacifolia (African Lettuce)
As an alternative to previously used, costly, and hazardous chemicals, nickel nanoparticles (NiNPs) were manufactured utilizing locally accessible biodiversity plants. The aim of this work is to examine the antibacterial capabilities of nickel nanoparticles generated from Launaea taraxacifolia (African lettuce or wild lettuce). Leaves of African lettuce (Launaea taraxacifolia) were gathered in Esa-Oke, Nigeria's Osun State. In the morning, a sterile container was used to collect urine. The urine sample was evaluated and cultured using C.L.E.D. medium, MacConkey agar, and Sabouraud dextrose agar. Traditional methods including as morphology, gram staining, biochemical analysis, and sugar fermentation were used to identify the isolates. African lettuce was cut into small pieces after being washed in distilled water. A nickel nanoparticle was made using a conventional approach from African lettuce leaf extract. Transmission Electron Microscopy was used to characterize the nanoparticle, which was done via a drop coating method (SEM). The isolates were identified as Klebsiella sp. and Micrococcus sp. using Bergy's manual for bacterial identification. Nickel nanoparticles and African lettuce leaf extract were tested for antibacterial activities against Klebsiella sp. and Micrococcus sp. utilizing agar well diffusion methods. The comprehensive Transmission Electron Microscopy (TEM) photos clearly demonstrate that nickel nanoparticles have identical morphologies and sizes, according to the findings of the study of generated nanoparticles. Nickel nanoparticles with a diameter distribution of 10–20 nanometers are visible in the TEM images. The zones of inhibition for nickel nanoparticles against tested bacterial isolates (Klebsiella sp and Micrococcus sp) were 2 mm and 0 mm, respectively, however the zones of inhibition for Launaea taraxacifolia extract were substantial (5 mm and 7 mm). Plant extracts (Launaea taraxacifolia) showed good inhibition against bacteria when compared to the stated zone of inhibition with generated nickel nanoparticles (Micrococcus sp and Klebisiella sp)
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