M. Asril
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引用次数: 2

摘要

吲哚乙酸(IAA)是促进植物生长的重要激素。它与氨基酸色氨酸有关,色氨酸是构建IAA的前体。而有些细菌如芽孢杆菌和大肠杆菌等在培养基上不需要l -色氨酸也能通过色氨酸独立途径产生IAA。本研究旨在确定本土芽孢杆菌和大肠杆菌分离物通过不依赖色氨酸的途径(不含L-色氨酸)生产IAA的潜力。本研究阶段包括分离物在不含色氨酸的液体培养基上生长,并采用Salkowsky法测定IAA水平。芽孢杆菌和大肠杆菌产生IAA的浓度分别为0,0236 mmol/L和0,024 mmol/L。这些细菌产生的IAA浓度很低,因为它们能够从独立的色氨酸途径合成IAA。独立的色氨酸途径意味着这些细菌可以合成IAA,而不需要在其生长培养基上使用色氨酸作为前体
本文章由计算机程序翻译,如有差异,请以英文原文为准。
UJI POTENSI Bacillus sp. DAN Escherichia coli DALAM MENGHASILKAN INDOLE ACETIC ACID (IAA) TANPA MENGGUNAKAN TRIPTOFAN PADA MEDIA PERTUMBUHAN
Indole Acetic Acid (IAA) is the important phytohormone for boosting plant-growth promotion. It is related with amino acid tryptophan as a precursor for building IAA. However some bacteria such as Bacillus sp. and Escherichia coli enable to produce IAA without L-tryptophan on culture medium via the tryptophan independent pathways. This study aims to determine the potential of indigenous Bacillus sp. and E. coli isolate in producing IAA via Trp-independent pathways (without L- tryptophan). The stage of this study consists of growing of isolate on liquid media without tryptophan and measuring the levels of IAA using Salkowsky method. Bacillus sp and Escherichia coli could produce IAA with the concentration of 0,0236 mmol/L and 0,024 mmol/L, respectively. The concentration of IAA produced by these bacteria is quite low because of their ability to synthesis IAA from the independent tryptophan pathway. The independent tryptophan pathway means these bacteria could synthesize IAA without using tryptophan as a precursor on their growth medium. n
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