SEM-CT observation of bone from mice using environmental-SEM

Computer topography (CT) imaging is one of the powerful-tools for nondestructive observation technique. In this paper, we present observation of bone from mice by scanning electron microscope (SEM)-CT system using environmental- SEM (E-SEM). The advantage of this SEM-CT system is able to image in high-resolution with less than 400 nm resolution. We investigated the effects of X-ray wavelengths and intensities to improve the imaging contrast for SEM-CT observation. From experimental results, a porous structures were observed inside the bone sample with approximately 10 μm pores.