{"title":"用铵离子检测法测定生物流体中尿素的EnFET","authors":"M. Dawgul, T. Trybun, D. Pijanowska, W. Torbicz","doi":"10.1117/12.517106","DOIUrl":null,"url":null,"abstract":"In this paper a method of urease immobilization on the surface of the Siloprene membrane of the ammonium ion sensitive ChemFET is presented. The usability of the sensor for determination of urea in solutions at pH typical for biological fluids (pH 6 to pH 7.5) has been investigated. Due to the fact that the sensor exhibits high sensitivity to samples of low buffer capacity, the method of preliminary sample treatment, consisting in addition of buffers at adequate buffer capacity was developed. The sensors were tested in dialysate and blood plasma.","PeriodicalId":405495,"journal":{"name":"Optoelectronic and Electronic Sensors","volume":"11 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2003-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"EnFET for urea determination in biological fluids using ammonium ion detection\",\"authors\":\"M. Dawgul, T. Trybun, D. Pijanowska, W. Torbicz\",\"doi\":\"10.1117/12.517106\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this paper a method of urease immobilization on the surface of the Siloprene membrane of the ammonium ion sensitive ChemFET is presented. The usability of the sensor for determination of urea in solutions at pH typical for biological fluids (pH 6 to pH 7.5) has been investigated. Due to the fact that the sensor exhibits high sensitivity to samples of low buffer capacity, the method of preliminary sample treatment, consisting in addition of buffers at adequate buffer capacity was developed. The sensors were tested in dialysate and blood plasma.\",\"PeriodicalId\":405495,\"journal\":{\"name\":\"Optoelectronic and Electronic Sensors\",\"volume\":\"11 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2003-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optoelectronic and Electronic Sensors\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1117/12.517106\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optoelectronic and Electronic Sensors","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/12.517106","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
EnFET for urea determination in biological fluids using ammonium ion detection
In this paper a method of urease immobilization on the surface of the Siloprene membrane of the ammonium ion sensitive ChemFET is presented. The usability of the sensor for determination of urea in solutions at pH typical for biological fluids (pH 6 to pH 7.5) has been investigated. Due to the fact that the sensor exhibits high sensitivity to samples of low buffer capacity, the method of preliminary sample treatment, consisting in addition of buffers at adequate buffer capacity was developed. The sensors were tested in dialysate and blood plasma.
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