8-OHdG and hOGG1 as Oxidative DNA Damage Markers in Acne Vulgaris Patients Under Isotretinoin Treatment

Background: Acne vulgaris is the most common inflammatory skin disease. It is primarily observed in adolescents and is characterized by comedones, papules, pustules, nodules, and cysts on the face, back, chest, chin, and body skin. Acne vulgaris affects about 80% of teenagers and continues beyond the age of 25 years in 3% of men and 12% of women in the world. Isotretinoin is one of themostcommontreatmentagentsforacnevulgaris, whichcausesoxidativeDNAdamageinthecell. Asanimportantindicator of oxidative DNA damage, 8-hydroxy-2’-deoxyguanosine is repaired with an enzyme called human 8-oxoguanine DNA glycosylase 1 (hOGG1). Objectives: WeaimedtoevaluateoxidativeDNAdamageinacnevulgarisbeforeandafterisotretinointreatmentbymeasuringthe 8-OHdG and hOGG1 levels. Methods: The8-OHdGandhOGG1levelswereevaluatedfromserumsamplesusingtheenzyme-linkedimmunosorbentassay(ELISA) method. Both the serum 8-OHdG (P < 0.05; P < 0.0001) and hOGG1 (P < 0.05; P = 0.04) levels were found to be statistically higher in the sixth month after isotretinoin treatment. Results: In this first report, the 8-OHdG and hOGG1 levels were found to be statistically significantly high after isotretinoin treatment. According to our results, the 8-OHdG level increased under isotretinoin administration in acne vulgaris patients. Conclusions: Consequently, healing via hOGG1 likely continues after dropping isotretinoin for DNA.