R.L.E. Ehrenkaufer, S. Klam, K. Makoroff, S. Giandinoto, T. Morton, D. Moroney, P. Nowak
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引用次数: 5
摘要
研究了未处理血浆样品的内表面反相色谱法(ISRP)作为定量动脉血浆代谢物[18F] n -甲基胡椒酮时间过程的替代方法。将ISRP法与目前广泛使用的标准固相萃取/高效液相色谱法(SPE/HPLC)进行了直接比较。结果表明:(1)ISRP方法快速，样品制备量少;(2) ISRP柱的放射性回收率为>90%;(3)没有放射性残留与色谱大小排除的蛋白质相关;(4)定量结果与传统的SPE/HPLC方法有很好的相关性。
Internal-surface reversed-phase chromatography for plasma metabolite analysis of radiopharmaceuticals
The use of internal-surface reversed-phase (ISRP) chromatography of unprocessed plasma samples was investigated as an alternative method of quantitation of the arterial plasma metabolite time course of [18F]N-methylspiperone. The ISRP method was directly compared to standard solid phase extraction/HPLC (SPE/HPLC) methods currently in wide use. Results indicate that: (1) the ISRP method is rapid and minimizes sample preparation; (2) recovery of radioactivity from the ISRP column is > 90%; (3) no radioactivity remains associated with chromatographically size excluded proteins and (4) the quantitative results are well correlated with conventional SPE/HPLC methods.
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