{"title":"[14C]氯络碱放射自显影法测定区域单胺氧化酶的简便方法","authors":"Matsutaro Murakami , Yasushi Kondoh , Yin Weimin , Sigenori Mizusawa , Hiroyuki Nakamichi , Kazuhiro Takahashi , Hiroshi Sasaki , Hidehiro Iida , Shuichi Miura , Iwao Kanno , Kazuo Uemura","doi":"10.1016/0883-2897(92)90096-H","DOIUrl":null,"url":null,"abstract":"<div><p>The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [<sup>14</sup>C]clorgyline in rat. [<sup>14</sup>C]Clorgyline was synthesized by the methylation reaction of <em>N</em>-desmethylclorgyline and [<sup>14</sup>C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.</p></div>","PeriodicalId":14328,"journal":{"name":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","volume":"19 6","pages":"Pages 619-623, 625-626"},"PeriodicalIF":0.0000,"publicationDate":"1992-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0883-2897(92)90096-H","citationCount":"4","resultStr":"{\"title\":\"A convenient method for regional monoamine oxidase-a determination by [14C]clorgyline autoradiography\",\"authors\":\"Matsutaro Murakami , Yasushi Kondoh , Yin Weimin , Sigenori Mizusawa , Hiroyuki Nakamichi , Kazuhiro Takahashi , Hiroshi Sasaki , Hidehiro Iida , Shuichi Miura , Iwao Kanno , Kazuo Uemura\",\"doi\":\"10.1016/0883-2897(92)90096-H\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [<sup>14</sup>C]clorgyline in rat. [<sup>14</sup>C]Clorgyline was synthesized by the methylation reaction of <em>N</em>-desmethylclorgyline and [<sup>14</sup>C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.</p></div>\",\"PeriodicalId\":14328,\"journal\":{\"name\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"volume\":\"19 6\",\"pages\":\"Pages 619-623, 625-626\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1992-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0883-2897(92)90096-H\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/088328979290096H\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Radiation Applications and Instrumentation. Part B. Nuclear Medicine and Biology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/088328979290096H","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
A convenient method for regional monoamine oxidase-a determination by [14C]clorgyline autoradiography
The availability of clorgyline for regional monoamine oxidase-A (MAO-A) determination was examined using [14C]clorgyline in rat. [14C]Clorgyline was synthesized by the methylation reaction of N-desmethylclorgyline and [14C]methyliodide in dimethylformamide with high radiochemical yield. The MAO-A distribution map by autoradiography correlated with that by histochemical technique and its quantity was consistent with the calculated MAO-A amount based on previous reports. The combination of labeled clorgyline and autoradiographic technique will promise the quantitative measurement of regional MAO-A distribution.
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