[不同条件下60Co γ射线照射下大鼠骨髓造血基质细胞前体(CFU-F)的放射敏感性]。

Radiobiologiia Pub Date : 1992-11-01
A I Kolesnikova, S Sh Kal'sina, A G Konopliannikov, L A Lepekhina
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引用次数: 0

摘要

克隆大鼠骨髓造血基质的前体细胞的条件已经开发出来,这种细胞在培养致密和稀疏的成纤维细胞菌落(CFU-F)中形成,其电泳效率为10(-4)。60co - γ辐照下大鼠骨髓CFU-F的放射敏感性,各克隆的Do和n值分别为1.87 Gy和1.4;密集无性系为0.65 Gy和6.7,稀疏无性系为4.27 Gy和1.0。这证实了观察到的CFU-F群体的异质性,包括高度辐射敏感和辐射耐药亚群体。在体内和体外辐照下,大鼠骨髓CFU-F参数基本一致;在原位辐照下,氧效应在CFU-F的辐射敏感亚群中起作用;所有克隆、密集克隆和稀疏克隆的OER值分别为1.6、2.6和0.9。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Radiosensitivity of cells-precursors of hemopoietic stroma (CFU-F) in the rat bone marrow under the effect of 60Co gamma irradiation in various conditions].

Conditions have been developed for cloning cells-precursors of rat bone marrow haemopoietic stroma, that form in culture dense and sparse fibroblast colonies (CFU-F) at a plating efficiency of 10(-4). Radiosensitivity of rat bone marrow CFU-F, with 60Co-gamma-irradiation in vitro, is characterized by the values of Do and n of 1.87 Gy and 1.4 respectively for all clones; 0.65 Gy and 6.7 for dense clones, and 4.27 Gy and 1.0 for sparse clones. This confirms the observed heterogeneity of CFU-F population consisting of highly radiosensitive and radioresistant subpopulations. The parameters of rat bone marrow CFU-F are nearly the same with irradiation both in vivo and in vitro; with in situ irradiation, the oxygen effect comes into play in a radiosensitive subpopulation of CFU-F; the OER values are 1.6, 2.6 and 0.9 for all, dense and sparse clones respectively.

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