[低剂量伽马辐射与人类细胞对诱变剂产生抗性的作用机制]。

Radiobiologiia Pub Date : 1992-11-01
T A Sinel'shchikova, G D Zasukhina
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引用次数: 0

摘要

用低剂量(0.07 ~ 0.21 Gy)的γ辐照人成纤维细胞。在短时间间隔(3 h)后,研究辐射后细胞的活力(通过台盼蓝染料排除法);后n -甲基-n '-硝基-亚硝基胍-DNA合成(诱变剂处理后立即加入3h -胸腺嘧啶)和后辐射诱导DNA单链断裂(通过在膜过滤器上裂解的细胞碱性洗脱)。用低剂量的伽马射线对细胞进行预照射,可以使细胞抵抗随后暴露于伽马辐射诱导的DNA断裂。成活率提高;与未辐照细胞相比,这些细胞的DNA合成对烷基化损伤具有抗性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[The mechanism of action of low doses of gamma radiation connected with the development of human cell resistance to mutagens].

The human fibroblasts were gamma-irradiated with low doses (0.07-0.21 Gy). After a short time interval (3 h), a study was made of the postirradiation viability of cells (by the trypan blue dye exclusion method); post-N-methyl-N'-nitro-nitrosoguanidine-DNA synthesis (by 3H-thymidine incorporation immediately after the mutagen treatment) and postirradiation induction of DNA single-strand breaks (by alkaline elution of cells lysed on the membrane filters). The preirradiation of cells with low doses of gamma-rays was shown to render the cells resistance to induction of DNA breaks by the following exposure to gamma-radiation. The survival rate increased; DNA synthesis was resistant to alkylation damage in these cells, as compared to nonirradiated cells.

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