模型细胞系统中巨大DNA和肌动蛋白丝之间的串扰

M. Negishi, T. Sakaue, K. Takiguchi, K. Yoshikawa
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引用次数: 0

摘要

我们将肌动蛋白和巨大的DNA混合物限制在磷脂包被的微米尺度(细胞大小)封闭空间(PMS)中作为细胞核模型系统。我们报道在肌动蛋白丝和巨大DNA分子之间的pms中出现空间分离状态。当PMS直径小于15 μm时,DNA分子通过与肌动蛋白丝形成组装而被排除在表面。此外,当肌动蛋白共存浓度足够高时,直径大于15 μm的PMS表面仍可观察到肌动蛋白和DNA的聚集。随着肌动蛋白浓度的降低,肌动蛋白丝倾向于溶解在球内,而DNA分子仍然被排除在表面。当肌动蛋白浓度变得更低时,DNA分子通过避免表面附着而溶解在球体内。我们将这些结果解释为在PMS中肌动蛋白丝表面附近产生的耗竭区。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Crosstalk between giant DNA and actin filament in a model cellular system
We confined actin and giant DNA mixture in a phospholipid coated micrometer scale (cell size) closed space (PMS) as a cell nuclear model system. We report the appearance of a spatially segregated state in PMSs between actin filaments and giant DNA molecules. When the diameter of a PMS was below 15 μm, DNA molecules are excluded toward the surface by forming an assembly with actin filaments. Furthermore, when the coexisting actin concentration was high enough, the aggregation of actin and DNA were still observed on the surface of a PMS whose diameter was above 15 μm. With a decrease in the actin concentration, actin filaments tend to dissolve within the sphere whereas DNA molecules remain to be excluded onto the surface. When the actin concentration becomes still lower, DNA molecules dissolve within the sphere by avoiding surface attachment. We interpreted these results in terms of the generation of a depletion zone near a surface with actin filaments in a PMS.
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