荧光光照对小檗离体微繁的影响

Kollawat Somsri, Pataradawn Pinyopich, W. Mohammed
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引用次数: 1

摘要

采用Philips TLD 36W/54和Toshiba FL40T8BRF/36两种不同类型的荧光光源,对Lemna minor stain SING-4的无性体外繁殖进行了研究。液体培养基中含有4.43gl-1无植物激素的全强度Murashige & Skoog (MS)基础培养基,含维生素、30gl-1蔗糖和1gl-1 MES。结果表明,两种植物的无性繁殖均正常,且呈指数增长趋势。与飞利浦TLD 36W/54(叶色图4)相比,东芝FL40T8BRF/36培养基的繁殖速度略快,同时表达出明显更绿的叶片(叶色图8号),这表明存在更多的叶绿素。从我们的实验中获得的数据表明,东芝FL40T8BRF/36发出的光产生更健康和更高质量的培养物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of fluorescent lighting on in vitro micropropagation of Lemna minor
The vegetative in vitro propagation of Lemna minor stain SING-4 exposed to two different types of fluorescent light sources, Philips TLD 36W/54 and Toshiba FL40T8BRF/36, was studied. The liquid culture medium contained 4.43gl-1 phytohormone-free full-strength Murashige & Skoog (MS) basal medium with vitamins, 30gl-1 sucrose, and 1gl-1 MES. The results showed that both plant cultures had undergone normal asexual reproduction with an exponential increase trend. Cultures exposed to Toshiba FL40T8BRF/36 reproduced at a slightly faster rate while expressing significantly greener foliage (leaf color chart shade No.8), which indicates the presence of more chlorophyll, than cultures exposed to Philips TLD 36W/54 (leaf color chart shade No.4). The data obtained from our experiment reveals that light emitted from Toshiba FL40T8BRF/36 produces healthier and higher quality cultures.
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