Kollawat Somsri, Pataradawn Pinyopich, W. Mohammed
{"title":"荧光光照对小檗离体微繁的影响","authors":"Kollawat Somsri, Pataradawn Pinyopich, W. Mohammed","doi":"10.1117/12.861349","DOIUrl":null,"url":null,"abstract":"The vegetative in vitro propagation of Lemna minor stain SING-4 exposed to two different types of fluorescent light sources, Philips TLD 36W/54 and Toshiba FL40T8BRF/36, was studied. The liquid culture medium contained 4.43gl-1 phytohormone-free full-strength Murashige & Skoog (MS) basal medium with vitamins, 30gl-1 sucrose, and 1gl-1 MES. The results showed that both plant cultures had undergone normal asexual reproduction with an exponential increase trend. Cultures exposed to Toshiba FL40T8BRF/36 reproduced at a slightly faster rate while expressing significantly greener foliage (leaf color chart shade No.8), which indicates the presence of more chlorophyll, than cultures exposed to Philips TLD 36W/54 (leaf color chart shade No.4). The data obtained from our experiment reveals that light emitted from Toshiba FL40T8BRF/36 produces healthier and higher quality cultures.","PeriodicalId":245973,"journal":{"name":"Southeast Asian International Advances in Micro/Nano-technology","volume":"18 4 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effects of fluorescent lighting on in vitro micropropagation of Lemna minor\",\"authors\":\"Kollawat Somsri, Pataradawn Pinyopich, W. Mohammed\",\"doi\":\"10.1117/12.861349\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The vegetative in vitro propagation of Lemna minor stain SING-4 exposed to two different types of fluorescent light sources, Philips TLD 36W/54 and Toshiba FL40T8BRF/36, was studied. The liquid culture medium contained 4.43gl-1 phytohormone-free full-strength Murashige & Skoog (MS) basal medium with vitamins, 30gl-1 sucrose, and 1gl-1 MES. The results showed that both plant cultures had undergone normal asexual reproduction with an exponential increase trend. Cultures exposed to Toshiba FL40T8BRF/36 reproduced at a slightly faster rate while expressing significantly greener foliage (leaf color chart shade No.8), which indicates the presence of more chlorophyll, than cultures exposed to Philips TLD 36W/54 (leaf color chart shade No.4). The data obtained from our experiment reveals that light emitted from Toshiba FL40T8BRF/36 produces healthier and higher quality cultures.\",\"PeriodicalId\":245973,\"journal\":{\"name\":\"Southeast Asian International Advances in Micro/Nano-technology\",\"volume\":\"18 4 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-05-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Southeast Asian International Advances in Micro/Nano-technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1117/12.861349\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Southeast Asian International Advances in Micro/Nano-technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/12.861349","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effects of fluorescent lighting on in vitro micropropagation of Lemna minor
The vegetative in vitro propagation of Lemna minor stain SING-4 exposed to two different types of fluorescent light sources, Philips TLD 36W/54 and Toshiba FL40T8BRF/36, was studied. The liquid culture medium contained 4.43gl-1 phytohormone-free full-strength Murashige & Skoog (MS) basal medium with vitamins, 30gl-1 sucrose, and 1gl-1 MES. The results showed that both plant cultures had undergone normal asexual reproduction with an exponential increase trend. Cultures exposed to Toshiba FL40T8BRF/36 reproduced at a slightly faster rate while expressing significantly greener foliage (leaf color chart shade No.8), which indicates the presence of more chlorophyll, than cultures exposed to Philips TLD 36W/54 (leaf color chart shade No.4). The data obtained from our experiment reveals that light emitted from Toshiba FL40T8BRF/36 produces healthier and higher quality cultures.