M. Taupik, Muhammad N. Allade, Mohammad Adam Mustapa, Endah Nurrohwinta Djuwarno, Jafar La Kilo
{"title":"Identifikasi dan karakterisasi senyawa alkaloid ekstrak metanol daun kecubung (datura metel l.) Dengan menggunakan Spektrofotometri Fourier Transform Infrared (Ft-Ir) Dan Spektrofotometri Uv-Visibel","authors":"M. Taupik, Muhammad N. Allade, Mohammad Adam Mustapa, Endah Nurrohwinta Djuwarno, Jafar La Kilo","doi":"10.31942/ce.v6i2.5501","DOIUrl":null,"url":null,"abstract":"The study aims to identify and characterize the alkaloid compounds of datura leaf (Datura metel L.) methanol extract by using Fourier Transform Infra Red (FT-IR) spectrophotometric and UV-Visible spectrophotometric. This study employs experimental methods including exctraction process, identication of compounds, thin layer chromatography, preparative thin- layer chromatography, and characterization of the compound by ultilizing UV-Vis spectrophotometer and IR spectrophotometer instruments. Furthermore, 150-gram datura leaves (Datura metel L.) are macerated with methanol solvents, and it resulting 20-gram methanol extracts. The result of thin layer chromatography using an eluent of chloroform : N-Hexane obtsins spot with the Rf Value of 2,07 ; O,56, 0,29 ; 3,5 ; 3,7 Beside, the result of the Uv_vis spectrophotometer shows that datura leaf isolates provide apsorption at wavelegenths of 234 and 284, which indicates the occurrence of n→σ* and n→π*. Electron transitions. At the same time, the data interoretation result using IR spectrophotometer of datura leaf (Datura metel L.) isolates indicate that there is an alkaloid compound with fuctional group of N-H (3301.354 cm-1) , Aromatic C-H (2940.153 ; 2830.842 cm-1), O=H (2044.644 cm-1), C-H (1448.258 cm-1), C-N (1114.566 cm-1), C-O (1000 cm-1).","PeriodicalId":387584,"journal":{"name":"CENDEKIA EKSAKTA","volume":"85 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"CENDEKIA EKSAKTA","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31942/ce.v6i2.5501","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Identifikasi dan karakterisasi senyawa alkaloid ekstrak metanol daun kecubung (datura metel l.) Dengan menggunakan Spektrofotometri Fourier Transform Infrared (Ft-Ir) Dan Spektrofotometri Uv-Visibel
The study aims to identify and characterize the alkaloid compounds of datura leaf (Datura metel L.) methanol extract by using Fourier Transform Infra Red (FT-IR) spectrophotometric and UV-Visible spectrophotometric. This study employs experimental methods including exctraction process, identication of compounds, thin layer chromatography, preparative thin- layer chromatography, and characterization of the compound by ultilizing UV-Vis spectrophotometer and IR spectrophotometer instruments. Furthermore, 150-gram datura leaves (Datura metel L.) are macerated with methanol solvents, and it resulting 20-gram methanol extracts. The result of thin layer chromatography using an eluent of chloroform : N-Hexane obtsins spot with the Rf Value of 2,07 ; O,56, 0,29 ; 3,5 ; 3,7 Beside, the result of the Uv_vis spectrophotometer shows that datura leaf isolates provide apsorption at wavelegenths of 234 and 284, which indicates the occurrence of n→σ* and n→π*. Electron transitions. At the same time, the data interoretation result using IR spectrophotometer of datura leaf (Datura metel L.) isolates indicate that there is an alkaloid compound with fuctional group of N-H (3301.354 cm-1) , Aromatic C-H (2940.153 ; 2830.842 cm-1), O=H (2044.644 cm-1), C-H (1448.258 cm-1), C-N (1114.566 cm-1), C-O (1000 cm-1).