M. Taupik, Muhammad N. Allade, Mohammad Adam Mustapa, Endah Nurrohwinta Djuwarno, Jafar La Kilo
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摘要

采用傅里叶变换红外(FT-IR)分光光度法和紫外-可见分光光度法对曼陀罗叶甲醇提取物的生物碱类化合物进行了鉴定和表征。本研究采用提取工艺、化合物鉴定、薄层色谱法、制备薄层色谱法、紫外可见分光光度计和红外分光光度计对化合物进行表征等实验方法。此外,150克曼陀罗叶(曼陀罗金属L.)用甲醇溶剂浸泡,得到20克甲醇提取物。以氯仿-正己烷为洗脱液的薄层色谱结果显示,样品的Rf值为2,07;0, 56, 0,29;3、5;3,7紫外-可见分光光度计结果表明,曼陀罗叶分离物在234和284波长处有吸附,说明存在n→σ*和n→π*。电子转换。同时,利用红外分光光度计对曼陀罗叶(datura metel L.)分离物进行数据分析,结果表明,该植物中含有官能团为N-H (3301.354 cm-1)、芳香族C-H (2940.153;2830.842 cm-1), O=H (2044.644 cm-1), C-H (1448.258 cm-1), C-N (1114.566 cm-1), C-O (1000 cm-1)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identifikasi dan karakterisasi senyawa alkaloid ekstrak metanol daun kecubung (datura metel l.) Dengan menggunakan Spektrofotometri Fourier Transform Infrared (Ft-Ir) Dan Spektrofotometri Uv-Visibel
The study aims to identify and characterize the alkaloid compounds of datura leaf (Datura metel L.) methanol extract by using Fourier Transform Infra Red (FT-IR) spectrophotometric and UV-Visible spectrophotometric. This study employs experimental methods including exctraction process, identication of compounds, thin layer chromatography, preparative thin- layer chromatography, and characterization of the compound by ultilizing UV-Vis spectrophotometer and IR spectrophotometer instruments. Furthermore, 150-gram datura leaves (Datura metel L.) are macerated with methanol solvents, and it resulting 20-gram methanol extracts. The result of thin layer chromatography using an eluent of chloroform : N-Hexane obtsins spot with the Rf Value of 2,07 ; O,56, 0,29 ; 3,5 ; 3,7 Beside, the result of the Uv_vis spectrophotometer shows that datura leaf isolates provide apsorption at wavelegenths of 234 and 284, which indicates the occurrence of n→σ* and n→π*. Electron transitions. At the same time, the data interoretation result using IR spectrophotometer of datura leaf (Datura metel L.) isolates indicate that there is an alkaloid compound with fuctional group of N-H (3301.354 cm-1) , Aromatic C-H (2940.153 ; 2830.842 cm-1), O=H (2044.644 cm-1), C-H (1448.258 cm-1), C-N (1114.566 cm-1), C-O (1000 cm-1).
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