黄连木属的SSR标记

S. Kafkas
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引用次数: 5

摘要

简单序列重复序列(SSRs)由于其在基因组中的丰度、共显性和高重复能力而成为最强大的分子标记系统之一。由于其可食用的坚果具有商业价值,因此它是开心果属的栽培物种。该属的其他种类是野生的,对于砧木资源以及观赏和森林树木尤其重要。直到几年前,关于黄连木的SSR标记数量还非常有限;然而,自2016年以来,下一代测序(NGS)技术已经允许在该属中开发出大量的SSRs。目前已发表的从栽培紫花苜蓿中开发的SSR标记约有1500个。也有一些研究从野生黄连木种中提取SSR位点。综上所述,目前已有足够数量的SSR标记用于栽培开心果,并且由于其在开心果种间具有较高的可转移率,可用于野生开心果种。这些ssr可用于黄连木属植物的自然种群多样性分析、标记发现、种质鉴定、亲本鉴定、遗传连锁作图和进化研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SSR Markers in the Genus Pistacia
Simple sequence repeats (SSRs) are one of the most powerful molecular marker systems due to abundance in the genomes, its codominant nature, and high repeat-ability. P. vera L. is cultivated species in the genus Pistacia due to commercial value of its edible nuts. Other species in the genus are in the wild and are important especially for rootstock sources as well as for ornamental and forest trees. There were a very limited number of SSR markers for Pistacia species until several years ago; however, next-generation sequencing (NGS) technology has allowed to develop plenty of SSRs since 2016 in the genus. There are currently about 1500 published SSR markers developed from cultivated P. vera . There are also several studies generating SSR loci from wild Pistacia species. In a conclusion, there are currently an adequate number of SSR markers for cultivated pistachio and that can be used in wild Pistacia species due to their high level of transferability rate between Pistacia species . These SSRs can be used for assaying diversity in natural populations, marker discovery, germplasm characterization, parental identification, genetic linkage mapping, and evolutionary studies in the genus Pistacia .
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