Development of molecular imprinted polymer interdigital sensor for C-terminal telopeptide of type I collagen

This paper presents a label-free and non-invasive technique for selective detection of C-terminal telopeptide type I collagen (CTx-I) by employing Electrochemical Impedance Spectroscopy to measure sample impedance. Molecular imprinted polymer, containing artificial recognition sites for CTx-was prepared by precipitation polymerization using CTx-I peptide as a template, methacrylic acid as a functional monomer and ethylene glycol methacrylate as the cross-linker. A high penetration depth planar interdigital sensor was functionalized by a self-assembled monolayer along with the synthesized MIP. Different concentrations of CTx-I sample solutions were tested using the proposed sensing system. High-Performance Liquid chromatography diode array system was used to validate the results.