硝酸盐对金属生物腐蚀的影响,由PLTA sasten细菌还原硫酸盐

N. Afianti, Dea Indriani Astuti
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引用次数: 0

摘要

硫酸盐还原菌对萨古岭水电站金属生物腐蚀中硝酸盐含量的影响。由微生物的活动促进和加速的腐蚀称为生物腐蚀。硫酸盐还原菌(Sulfate reducing bacteria, SRB)是一种在厌氧条件下以硫酸盐作为最终电子受体而引起生物腐蚀的细菌。在工业中,生物腐蚀降低了设备的使用寿命,增加了维护成本。在萨古岭水电站的冷却系统中,由于使用污染水而造成的腐蚀是常见的,因为污染水是由无机和有机废物,特别是硫酸盐引起的。本研究从萨古岭水电站冷却系统的生物膜中分离出硫酸盐还原菌。以dsrB基因(350 bp)为分子标记,采用PCR-DGGE方法进行分子分析,结果表明SRB联合体包含12个条带,推测为SRB的不同种。测试了SRB联合体对ST37(碳钢)和SUS304(不锈钢)金属材料的生物腐蚀活性。然后用7种不同浓度的硝酸盐处理该联合体,以确定其对硫酸盐还原菌活性的影响。SRB联合体对ST37的腐蚀速率高于SUS304L,腐蚀速率分别为0.07660 mm/年和0.00265 mm/年。10 mM的硝酸盐浓度有效抑制了ST37的腐蚀速率,并引起硫酸盐还原菌群落的变化,DGGE剖面中有6条条带消失
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pengaruh Nitrat terhadap Biokorosi Logam oleh Konsorsium Bakteri Pereduksi Sulfat dari PLTA Saguling
The Influence of Nitrate in Metal Biocorrosion caused by Sulfate Reducing Bacteria from Saguling Hydropower. The corrosion facilitated and accelerated by the activities of microorganism is called biocorrosion. Sulfate reducing bacteria (SRB) is known as the bacteria that cause biocorrosion in anaerobic condition by using sulfate as the final electron acceptor. Biocorrosion reduces equipment lifetime and increases maintenance cost in industry. In the cooling system in Saguling hydropower, corrosion was commonly caused by utilization of contaminated water due to anorganic and organic waste, especially sulfate. In this research, sulfate reducing bacteria was isolated from biofilms in the cooling system of Saguling Hydropower. Molecular analysis using PCR-DGGE method with dsrB gene (350 bp) as molecular markers showed that SRB consortium contained 12 bands and assumed as different species of SRB. SRB consortium was tested to determine its biocorrosion activity over metal material of ST37 (carbon steel) and SUS304 (stainless steel). The consortium then treated with 7 different nitrate concentrations to determine its effect against the sulfate reducing bacteria activity. SRB consortium caused higher corrosion to ST37 than SUS304L, with the corrosion rate of 0.07660 mm/year and 0.00265 mm/year, respectively. Concentration of 10 mM nitrate effectively inhibited corrosion rate on ST37 and caused the changes in sulfate reducing bacteria communities, indicated by the disappearance of 6 bands in DGGE profile
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