O. Bulteel, N. Van Overstraeten-Schlogel, P. Dupuis, D. Flandre
{"title":"完整的微系统使用SOI光电二极管DNA浓度测量","authors":"O. Bulteel, N. Van Overstraeten-Schlogel, P. Dupuis, D. Flandre","doi":"10.1109/BIOCAS.2010.5709591","DOIUrl":null,"url":null,"abstract":"In this paper, we describe a complete microsystem allowing the measurement of DNA concentration based on ultraviolet (UV) absorption. The system includes an ultraviolet light-emitting diode (LED) as light source and a silicon-on-insulator (SOI) lateral PIN diode as photodetector. After demonstrating the feasibility of the system with a quartz container, measurements are performed on DNA samples in PCR tubes by direct trans-mittance. The measurement of the sample in the tubes implies no waste neither manipulation of the samples. We study the impact of variation of the different parameters of the system, i.e. the wavelength of the LED, the light power reaching the samples and the bias of the photosensor. We are able to measure responses for DNA concentrations in the range from 400 ng/μL to 4 pg/μL and correlate bacteria concentrations to the induced photocurrent of the diode from 6.1011 spores/mL to 6.107 spores/mL. The system features a present precision of current measurements of 2%. In the optimal case, a limit of detection (LOD) of 0.02 ng/μL has been estimated.","PeriodicalId":440499,"journal":{"name":"2010 Biomedical Circuits and Systems Conference (BioCAS)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2010-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Complete microsystem using SOI photodiode for DNA concentration measurement\",\"authors\":\"O. Bulteel, N. Van Overstraeten-Schlogel, P. Dupuis, D. Flandre\",\"doi\":\"10.1109/BIOCAS.2010.5709591\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In this paper, we describe a complete microsystem allowing the measurement of DNA concentration based on ultraviolet (UV) absorption. The system includes an ultraviolet light-emitting diode (LED) as light source and a silicon-on-insulator (SOI) lateral PIN diode as photodetector. After demonstrating the feasibility of the system with a quartz container, measurements are performed on DNA samples in PCR tubes by direct trans-mittance. The measurement of the sample in the tubes implies no waste neither manipulation of the samples. We study the impact of variation of the different parameters of the system, i.e. the wavelength of the LED, the light power reaching the samples and the bias of the photosensor. We are able to measure responses for DNA concentrations in the range from 400 ng/μL to 4 pg/μL and correlate bacteria concentrations to the induced photocurrent of the diode from 6.1011 spores/mL to 6.107 spores/mL. The system features a present precision of current measurements of 2%. In the optimal case, a limit of detection (LOD) of 0.02 ng/μL has been estimated.\",\"PeriodicalId\":440499,\"journal\":{\"name\":\"2010 Biomedical Circuits and Systems Conference (BioCAS)\",\"volume\":\"7 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2010 Biomedical Circuits and Systems Conference (BioCAS)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/BIOCAS.2010.5709591\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2010 Biomedical Circuits and Systems Conference (BioCAS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/BIOCAS.2010.5709591","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Complete microsystem using SOI photodiode for DNA concentration measurement
In this paper, we describe a complete microsystem allowing the measurement of DNA concentration based on ultraviolet (UV) absorption. The system includes an ultraviolet light-emitting diode (LED) as light source and a silicon-on-insulator (SOI) lateral PIN diode as photodetector. After demonstrating the feasibility of the system with a quartz container, measurements are performed on DNA samples in PCR tubes by direct trans-mittance. The measurement of the sample in the tubes implies no waste neither manipulation of the samples. We study the impact of variation of the different parameters of the system, i.e. the wavelength of the LED, the light power reaching the samples and the bias of the photosensor. We are able to measure responses for DNA concentrations in the range from 400 ng/μL to 4 pg/μL and correlate bacteria concentrations to the induced photocurrent of the diode from 6.1011 spores/mL to 6.107 spores/mL. The system features a present precision of current measurements of 2%. In the optimal case, a limit of detection (LOD) of 0.02 ng/μL has been estimated.
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