真菌拮抗剂对豌豆根腐病的体外生物药效研究

Safdar Kaiser Hasmi, R. Khan
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引用次数: 0

摘要

采用马铃薯葡萄糖琼脂双培养技术,研究了黑曲霉、黄曲霉、康宁木霉、atroviride霉和harzianum霉对茄枯丝核菌的体外拮抗效果。5 d后的观察结果显示,各处理均优于对照,但拮抗效果最好的是黑曲霉,对病原菌的径向生长抑制率最高(77.01%),其次是黄曲霉、哈兹黑曲霉和科宁曲霉,分别为66.23%、64.42%和62.20%。结果表明,阿托维脲的抑菌效果最差,抑菌率为42.21%。与此同时,对照菌(solani R.)呈100%径向生长,并在5天内覆盖整个培养皿。除atroviide能有效抑制菌核形成外,其余药剂均能有效抑制菌核形成,其余药剂均能在10 d后完全抑制菌核形成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-vitro bioefficacy of fungal antagonists against root rot of Pea caused by Rhizoctonia solani (Kuhn)
In-vitro effectiveness of various antagonistic fungi namely Aspergillus niger, A. flavus, Trichoderma koningii, T. atroviride, and T. harzianum were evaluated against Rhizoctonia solani by dual culture technique on potato dextrose agar. According to the observation recorded after 5 days, all the treatments were found to be superior over control (R. solani), but among all treatments A. niger was found to the most effective antagonist, with highest radial growth inhibition of the pathogen (77.01 percent), followed by A. flavus, T. harzianum and T. koningii i.e., 66.23, 64.42 and 62.20 percent. While as, T. atroviride was found to be the least effective one with minimum growth inhibition i.e., 42.21 percent. Whereas, at the same time control (R. solani) showed 100 percent radial growth and covered the whole Petri Plate within 5 days. All the bio-control agents were significantly effective to inhibit the sclerotia formation and development, except T. atroviride in which formation of sclerotia was recorded, while in all other treatments complete inhibition of sclerotia formation was recorded after 10 days of incubation.
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