Cytotoxic Effects of Ceteareth-20 and Paraffinium Liquidum In Vitro

Aim: The aim of this study is to investigate the effects of ceteareth-20 and paraffinium liquidium on cell viability and cytotoxicity in human lymphocyte in vitro . Methods: We studied the cytotoxic and inhibitory effects of ceteareth-20 and paraffinium liquidum on cell proliferation using lactate dehydrogenase (LDH) assay and cell proliferation (WST-1) assay. Results: The cytotoxicity was enhanced when cells were treated with 1%, 5%, 25% and 50% paraffinium liquidium dilutions (p < 0.05). Moreover, cell number significantly reduced after 24 hours when they were treated with the same concentrations of paraffinium liqudium (p < 0.05). On the other hand, ceteareth-20 at concentrations at 1%, 5%, 25% and 50% showed no significant cytotoxic effect. These results showed that paraffinium liquidum dilutions have cytotoxic and proliferative effect otherwise ceteareth-20 dilutions have only proliferative effects on cultured human lymphocytes. Conclusion: It can be said that paraffinium liquidum and ceteareth-20 is harmful to use in beauty and cosmetic products. Future studies might allow alternative agents to be used instead of paraffinium liquidum and ceteareth-20.