S. Khaled, ,. M. A. Hammam, F. El-Shouny, S. El-Sayed, M. Aly-Aldin
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引用次数: 0
摘要
本研究旨在研究绿茶中酚类物质的含量,评价植物提取物的抗氧化活性,研究绿茶提取物对抗氧化应激的潜在保护作用,研究植物对纸杯蛋糕油化学性质的影响。该绿茶的主要成分为没食子酸甲酯,所研究的提取物具有体外和体内抗氧化活性,绿茶甲醇提取物在浓度为100µg/ml时还原力测定为0.645。在DPPH实验中,绿茶甲醇提取物在100µg/ml浓度下的抑制率为90.4%,而抗坏血酸的抑制率为97.7%。绿茶对肝功能(ALT和AST活性、ALB和TP水平)、肾功能(尿素和肌酐水平)和抗氧化指标(CAT活性和MDA水平)均有生物学效应。绿茶提取物治疗导致降低ALT和AST活性,增加血清总蛋白和白蛋白水平伴随着降低肌酐和尿素的水平,与此同时治疗H 2 O 2导致猫活性和MDA水平但海拔绿茶提取物治疗这个海拔高度的影响,也影响绿茶食品技术存储期间的蛋糕和影响下蛋糕油的酸值和过氧化值研究。
Biochemical and technological studies on green tea
: The present study was designed to investigate the phenolic contents in green tea, to evaluate the antioxidant activity of plant extract and to studying the potential effect of green tea extract to increase protective effect against oxidative stress, and study the effect of plant on chemical properties of oil used in cupcakes. The main component of such green tea was methyle gallate, the extracts under study was found to have antioxidant activity in vitro and in vivo, green tea methanol extract recorded 0.645 at concentration of 100µg/ml in reducing power assay. In DPPH assay green tea methanol extract recorded inhibition percent of 90.4% at concentration of 100 µg/ml comparing with ascorbic acid 97.7%. Green tea have biological effect on liver functions (ALT and AST activity, ALB and TP levels), kidney functions (urea and creatinine levels) and antioxidant markers (CAT activity and MDA level). The treatment with green tea extracts led to reduction in ALT and AST activity, and an increase in total protein and albumin levels accompanied by reduction in creatinine and urea levels, meanwhile treatment with H 2 O 2 caused an elevation in CAT activity and MDA level but treatment with green tea extracts affected this elevation, also green tea effected on food technology during storage period of cupcakes and effected on the acid value and peroxide value of cupcakes oil under study.