Triticum aestivum response to Xanthobacter autotrophicus and Bacillus thuringiensis optimized by crude extract carbon nano particles and 50% of NH4 NO3

Triticum aestivum requires nitrogen fertilizer (NF) as NH4NO3 for healthy growth, however its excessive application causes loss of soil fertility. An alternative to decrease the amount of NH4NO3 and to optimize is to inoculate T. aestivum seeds with Xanthobacter autotrophicus or/and Bacillus thuringiensis well known asendophytic plant growth promoting bacteria (EPGPB). Both genera and species of EPGPB are able transform metabolic compounds from seeds and roots into phyto hormones, which can be optimized by crude extract carbon nano particles (CECNPs) to enhance the growth of radical system and to improve uptake at 50% dose of NH4NO3 Response variables were: germination percentage, phenology, and biomass to seedling stage. All experimental data were validated by ANOVA/Tukey HSD P<0.05. Seeds of T. aestivum were inoculated with X. autotrophicus and/or B. thuringiensis, plus CECNPs at 50% NH4NO3. Results showed a positive response of T. aestivum seeds to X. autotrophicus and/or B. thuringiensis, with 10 ppm CECNPs, and 50% NH4NO3 enhanced germination percent of 93% in comparison with 73% of T. aestivum when its seeds were not inoculated with these EPGPB fed only with 100% dose of NH4NO3 (relative control); the same positive response of T. aestivum to X. autotrophicus and B. thuringiensis improved by CECNPs at seeding stage compared to T. aestivum used as a relative control.