{"title":"飞秒脉冲序列在(辐照度/辐照剂量)参数空间中的自由电子介导效应(会议报告)","authors":"N. Linz, Xiao-Xuan Liang, S. Freidank, A. Vogel","doi":"10.1117/12.2525153","DOIUrl":null,"url":null,"abstract":"Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.","PeriodicalId":157928,"journal":{"name":"Optical Interactions with Tissue and Cells XXX","volume":"20 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Free electron mediated effects of femtosecond pulse series in the (irradiance/irradiation dose) parameter space (Conference Presentation)\",\"authors\":\"N. Linz, Xiao-Xuan Liang, S. Freidank, A. Vogel\",\"doi\":\"10.1117/12.2525153\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.\",\"PeriodicalId\":157928,\"journal\":{\"name\":\"Optical Interactions with Tissue and Cells XXX\",\"volume\":\"20 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-03-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Optical Interactions with Tissue and Cells XXX\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1117/12.2525153\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Optical Interactions with Tissue and Cells XXX","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/12.2525153","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Free electron mediated effects of femtosecond pulse series in the (irradiance/irradiation dose) parameter space (Conference Presentation)
Understanding free-electron mediated effects of tightly focused femtosecond pulse series is essential for minimizing photodamage in nonlinear microscopy and opens new avenues for nanosurgery and intentional modifications of biomolecules. We tracked different stages of the photomodification kinetics (hyperfluorescence, plasma luminescence, bubble formation) by time-lapse 2-photon microscopy, fluorescence lifetime measurements, and bubble interferometry with nanometer resolution. Monitoring of bubble growth during pulse series enabled us to quantify chemical reaction rates leading to gas formation via molecular disintegration. Novel ways of data evaluation were used to create a comprehensive picture of the photomodification kinetics in the (irradiance/irradiation dose) parameter space.
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