Steroidal saponins isolation of Agave americana subsp. andina by enzymatic hydrolysis: a new approach.

. Nowadays, researching of steroidal saponins (SE) is not present in Ecuador, a country where there is a less the science investment; in spite of owning endemic flora unexplored like Agave americana subps. andina (or the famous “penco azul”); it is a great specie for phytochemical and biotechnician studies. On the other hand, China has been the major SE productor since the end of twentieth century, and actuality, it progresses in prodrugs enhance. For this reason, the author of this short-paper explains how Ecuadorian biotechnicians can do an experimental design with a novel method of enzymatic hydrolysis. This innovative procedure uses a potato-dextrose subtract, which is a culture medium for yeasts of chaguarmishki. This method is useful for extracting O-glucuronidase, an enzyme that catalyze O-glycosides. These molecules are the active agent on Agave genus. Finally, the importance of this research is not for the advantages of SE on pharmaceutics or its human body effect; really, the principal benefit is the enhance of Ecuadorian pharmaceutical industry through a mass production with an investment on science investigation.