Quantification of β-Sitosterol in Sesbania grandiflora Bark using High Performance Thin Layer Chromatography

: An efficient high performance thin layer chromatographic (HPTLC) method was optimized for the purpose of determining the levels of β -sitosterol (BS) in the bark of Sesbania grandiflora . The chromatographic separation was finished with a toluene: ethyl acetate: formic acid (50:15:5) mobile phase, and a densitometric scan was performed at 254 nm between 2 and 10 µg/mL. The developed strategy was linear, and the correlation coefficient was 0.9901. This study created and proved a straightforward HPTLC method that is precise, quick, selective, and simple for identifying and quantifying the phytochemical marker BS in S. grandiflora bark. The methanolic extract of S. grandiflora bark was determined to have a BS content of 153.09 µg/mg upon further investigation. The approach that was described above can be utilized to screen the presence of BS in herbal or pharmaceutical formulations, with potential applications in various industries. in methanolic extract of S. grangiflora bark that is both the simplest, most cost-effective, and most time-efficient approach currently available. This study will serve as a foundation upon which to build future investigations into such topics as precise identity verification, visual detection of well-separated phytoconstituents, and genuine medical applications. The main new things about this study are how easy it is to prepare samples; how fast this optimized fingerprinting method can give results; how consistent the results are; how cheap the method is; and how only one plate run is needed to evaluate plant parts. The goal of this study was to create a high-performance thin-layer chromatography (HPTLC) method for estimating BS from a single chromatogram. The BS content of the S. grandiflora bark extract was estimated using the established HPTLC technique.