利用碘化丙啶和吖啶橙同时鉴定枯草芽孢杆菌MZ的健康细胞和死亡细胞

Naieli Herrera-Reyes, V. Olalde‐Portugal, L. Sánchez-Segura
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引用次数: 0

摘要

微生物在许多工艺中的应用带来了新技术的发展。为了保证微生物的效果和影响,甚至当这些微生物参与生产特定化合物时的生产速度,有必要了解这种微生物的数量和条件。在现实中,有很多工具来识别这种情况,因为染色细胞试剂盒,直到最好的显微镜技术。但是,由于成本高,这种工具的使用可能变得难以获得,并且在去染试剂盒的情况下,测试有限。然后出现了搜索和实施新闻方法的必要性,以帮助我们识别不同接种剂或产品中的健康细胞和死亡细胞。在这项工作中,我们提出了一种利用白炽灯和紫外光的光学显微镜,通过碘化丙啶和吖啶橙染色样品同时观察健康细胞和死亡细胞的新技术。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identify healthy and dead cells simultaneity of Bacillus subtilis MZ through to use of propidium iodide and acridine orange
The use of microorganism in a lot of process brings the development of new technologies. For guarantee the effect and the impact of the microorganism, even, the production rate when these microorganisms are involved in the production of a particular compound, become necessary know the number and condition of this organism. In the actuality, there are a lot of tools to identify this condition, since kits of staining cells, until, the best microscopy technology. But the use of this tools could become inaccessible due the high cost and in the case of de staining kits had a limited test. Then emerge the necessity to search and implement news methodologies to help us to identify health and death cells in different inoculs or products. In this work, we proposed a new technology for the simultaneous observation of healthy and dead cells trough the staining samples with propidium iodide and acridine orange using an optic microscopy with incandescent light and ultraviolet light.
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