介质电泳颗粒/细胞分选微通道垂直电极的设计与制备

L. Wang, S. Marchenko, J. Huang, N. Jeon, E. Monuki, L. Flanagan, A.P. Lee
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引用次数: 1

摘要

我们已经开发了一种在微通道侧壁制造垂直电极的工艺。通过适当的电极设计,可以沿通道的横向方向产生DEP力,从而可以沿通道的宽度尺寸定位参与者。采用CFD-ACE软件进行有限元模拟,研究了不同电极配置对产生非均匀电场分布效果的影响。设计并研究了不同长度比电极和交叉电极对电场不均匀性的产生。与不同长度比的电极相比,侧壁交错电极提供更一致的DEP力方向。用DEP处理聚苯乙烯微珠和HEK293细胞的实验验证了该设计的性能。颗粒/细胞可以集中在通道的中间,被困在侧壁上,并被水流分选到不同的出口
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Design and Fabrication of Vertical Electrodes in Microchannels for Particles/cells Sorting by Dielectrophoresis
We have developed a process to fabricate vertical electrodes in the side walls of the microchannel. With appropriate electrodes design, DEP force along the lateral direction of the channel can be generated so that one can position participates along the width dimension of the channel. The effect of different electrode configurations on the efficacy of generating non-uniform electric field distribution has been studied by FEM simulation with CFD-ACE. Electrodes with various length ratios and interdigited electrodes have been designed and studied on the generation of non-uniformity of the electric field. Compared to electrodes with different length ratios, side-wall interdigited electrodes provide more consistent direction of DEP force. Experiments on the manipulation of polystyrene microbeads and HEK293 cells with DEP have been demonstrated to verify the performance of the design. The particles/cells can be focused in the middle of the channel, trapped on the side walls, and sorted to different outlets by the flow
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