用于测量与癌症相关的微小rna的纳米材料生物传感器的制造

2020 27th National and 5th International Iranian Conference on Biomedical Engineering (ICBME) Pub Date : 2020-11-26 DOI:10.1109/ICBME51989.2020.9319450

Homayoon Soleimani Dinani, Mehrab Pourmadadi, H. Rashedi, F. Yazdian

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引用次数: 10

摘要

描述了一种电化学纳米生物传感器，用于伏安法测定作为癌症生物标志物的miRNA-128。通过金纳米颗粒/磁铁矿/还原氧化石墨烯(AuNPs/Fe3O4/RGO)将适配体链固定在玻碳电极(GCE)表面。利用FTIR和XRD对合成的纳米材料进行了表征。采用方波伏安法(SWV)对改性GCE进行无标记表征。结果表明，该修饰电极对miRNA-128具有较高的选择性。六氰高铁酸盐氧化还原体系通常工作在0.3 V左右(相对于Ag/AgCl)，用作电化学探针。检测限(LOD)为0.05346 fM，线性检测范围为0.1 ~ 0.9 fM。

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Fabrication of nanomaterial-based biosensor for measurement of a microRNA involved in cancer

An electrochemical nanobiosensor is described for the voltammetric determination of miRNA-128 as the cancer biomarker. Aptamer chains were immobilized on the surface of a glassy carbon electrode (GCE) via gold nanoparticles/magnetite/reduced graphene oxide (AuNPs/Fe3O4/RGO). FTIR and XRD analysis were used for the characterization of synthesized nanomaterials. Square wave voltammetry (SWV) was used to characterize the modified GCE in a label-free method. The results indicate that the modified working electrode has high selectivity and for miRNA-128 over other biomolecules. The hexacyanoferrate redox system, typically operated at around 0.3 V (vs. Ag/AgCl) was used as an electrochemical probe. The limit of detection (LOD) and linear detection range are 0.05346 fM and 0.1 to 0.9 fM, respectively.

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2020 27th National and 5th International Iranian Conference on Biomedical Engineering (ICBME)

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