CRISPR系统:从适应性免疫到基因组编辑

The Promise of Science Pub Date : 2017-12-27 DOI:10.1142/S2529732517400090

J. Doudna

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引用次数: 0

摘要

CRISPR-Cas9是一种革命性的基因组编辑工具。了解Cas9如何识别DNA以及如何控制其功能对于改进该系统至关重要。我们使用单分子FRET来阐明DNA靶识别过程中的关键验证步骤。我们还使用x射线晶体学来显示Cas9抑制剂如何能够允许DNA结合但防止切割。最后，CRISPR研究不仅因其令人兴奋的应用而引人注目，还因其深刻的伦理含义而引人注目。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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CRISPR System: From Adaptive Immunity to Genome Editing

CRISPR-Cas9 is a revolutionary genome-editing tool. Understanding how Cas9 recognizes DNA and how to control its function will be critical in improving the system. We used single-molecule FRET to elucidate a key validation step during DNA target recognition. We also used X-ray crystallography to show how a Cas9 inhibitor is able to permit DNA binding but prevent cleavage. Finally, CRISPR research is notable not just for the exciting applications, but also for its profound ethical implications.

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The Promise of Science

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