巴亚尔塔港阿科斯海底峡谷上中光带海胆(Hesperocidaris asteriscus)的可培养细菌微生物群

e-CUCBA Pub Date : 2023-06-29 DOI:10.32870/ecucba.vi20.306
Paola Berenice Hernández-Vázquez, Héctor Ocampo-Alvarez, Cristian Moisés Galván-Villa, E. Ríos-Jara, Amayaly Becerril-Espinosa
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引用次数: 0

摘要

海洋生物,如海胆,孕育共生细菌,产生具有抗生素活性的代谢物。这些代谢物可以赋予宿主防御病原体的机制,有助于其生态适应性,也可以用于生物技术目的。因此,本研究的目的是分离海胆Hesperocidarisasteriscus的可培养菌群,并对其进行分析,寻找具有抗菌活性的菌株。在四种分离培养基中分离出该细菌,并通过对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌三种病原体的生长抑制来评估其抗生素活性。结果发现29株菌落形态各异,其中9株对至少一种病原菌具有生物活性。其中4个具有生物活性的菌株表现出放线菌门的典型形态特征。由此可见,生物活性细菌能够抑制海胆病原体的生长,从而有助于海胆的防御。然而,重要的是继续研究这些代谢物的搜索和表征,因为它们可以用于治疗对目前已知药物耐药的致病菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiota bacteriana cultivable del erizo de mar Hesperocidaris asteriscus, habitante de la zona mesofótica superior del cañón submarino de los Arcos, Puerto Vallarta
Marine organisms, such as sea urchins, harbor symbiotic bacteria that produce metabolites with antibiotic activity. Thesemetabolites can confer to the host a defense mechanism against pathogens, contributing to its ecological suitability, and can also beused for biotechnological purposes.Considering the above, the objective of this work was to isolate the culturable microbiota of the sea urchin Hesperocidarisasteriscus and analyze it in search of strains with antibiotic activity. The bacteria were isolated in four isolation media and theantibiotic activity was evaluated by growth inhibition in three pathogens: S. aureus, E. coli, and P. aeruginosa. As a result, 29strains with different colonial morphology were recovered and nine of these strains presented bioactivity against at least onepathogen. Four of these bioactive strains presented morphological attributes typical of the phylum Actinobacteria. It is concludedthat bioactive bacteria could contribute to the defense of the sea urchin, by being able to inhibit the growth of its pathogens.However, it is important to continue this line of research towards the search and characterization of these metabolites, since theycould be used for the treatment of pathogenic bacteria resistant to currently known drugs.
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