{"title":"一种电酶法柔性乳酸分子传感器","authors":"N. Thomas, I. Lähdesmäki, B. Parviz","doi":"10.1109/NEMS.2012.6196802","DOIUrl":null,"url":null,"abstract":"We present the fabrication and electrochemical characterization of an electronic molecular lactate sensor comprised of an optimized three-electrode setup on a transparent, flexible polymer substrate. The sensors are functionalized with the flavoenzyme lactate oxidase as a selective, nanoscale recognition element. They exhibit an exceptionally high sensitivity in their linear range of ~ 370 μAmM-1cm-2, a very low detection limit of ~ 5 μM, and good thermal stability, with a working electrode area of only ~ 0.19 mm2. We envision the sensors being utilized for one-time measurements of lactate levels in food or agricultural products for monitoring of their freshness, and show results of lactate level measurements for various dairy products.","PeriodicalId":156839,"journal":{"name":"2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2012-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"An electro-enzymatic flexible molecular lactate sensor\",\"authors\":\"N. Thomas, I. Lähdesmäki, B. Parviz\",\"doi\":\"10.1109/NEMS.2012.6196802\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"We present the fabrication and electrochemical characterization of an electronic molecular lactate sensor comprised of an optimized three-electrode setup on a transparent, flexible polymer substrate. The sensors are functionalized with the flavoenzyme lactate oxidase as a selective, nanoscale recognition element. They exhibit an exceptionally high sensitivity in their linear range of ~ 370 μAmM-1cm-2, a very low detection limit of ~ 5 μM, and good thermal stability, with a working electrode area of only ~ 0.19 mm2. We envision the sensors being utilized for one-time measurements of lactate levels in food or agricultural products for monitoring of their freshness, and show results of lactate level measurements for various dairy products.\",\"PeriodicalId\":156839,\"journal\":{\"name\":\"2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-03-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/NEMS.2012.6196802\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 7th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/NEMS.2012.6196802","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
摘要
我们提出了一种乳酸盐电子分子传感器的制造和电化学表征,该传感器由优化的三电极设置组成,位于透明、柔性的聚合物衬底上。该传感器是功能化与黄酶乳酸氧化酶作为一个选择性的,纳米级识别元件。它们在~ 370 μ am -1cm-2的线性范围内具有极高的灵敏度,极低的检测限为~ 5 μM,并且具有良好的热稳定性,工作电极面积仅为~ 0.19 mm2。我们设想传感器用于一次性测量食品或农产品中的乳酸水平,以监测其新鲜度,并显示各种乳制品的乳酸水平测量结果。
An electro-enzymatic flexible molecular lactate sensor
We present the fabrication and electrochemical characterization of an electronic molecular lactate sensor comprised of an optimized three-electrode setup on a transparent, flexible polymer substrate. The sensors are functionalized with the flavoenzyme lactate oxidase as a selective, nanoscale recognition element. They exhibit an exceptionally high sensitivity in their linear range of ~ 370 μAmM-1cm-2, a very low detection limit of ~ 5 μM, and good thermal stability, with a working electrode area of only ~ 0.19 mm2. We envision the sensors being utilized for one-time measurements of lactate levels in food or agricultural products for monitoring of their freshness, and show results of lactate level measurements for various dairy products.