腹水中富含淀粉样蛋白增强因子的巨噬细胞在腹腔内形成淀粉样蛋白。

K Miura, S Baba, H Shirasawa, S T Ju, A S Cohen, T Shirahama
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引用次数: 0

摘要

虽然来自淀粉样变小鼠的腹膜细胞(淀粉样腹膜细胞)能够将继发性淀粉样变的前体蛋白,血清淀粉样蛋白A (SAA)加工成淀粉样原纤维,但腹膜是淀粉样蛋白沉积的罕见部位。这被认为是由于腹膜中SAA的缺乏。为了增加SAA向腹膜的供应,在小鼠体内诱导了含有与血清中相同蛋白质成分的腹水。淀粉样腹膜细胞被包裹在过滤膜屏蔽的微室中,并在补充了额外炎症因子的腹水中培养。第7天,箱体内外均可见刚果红阳性结构,在偏振光下呈绿色双折射。通过抗aa或-SAA免疫染色,巨噬细胞的淀粉样蛋白沉积和细胞表面呈阳性。来自淀粉样腹膜细胞的T淋巴细胞和b淋巴细胞的免疫耗损对微室中淀粉样蛋白的形成没有不利影响。这些结果表明,腹水中含有足量的SAA或具有高淀粉样蛋白增强因子(AEF)活性的腹膜巨噬细胞是腹膜中AA淀粉样蛋白纤维形成所必需的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Intraperitoneal amyloid formation by amyloid enhancing factor--rich macrophages in ascitic fluid.

Although resident peritoneal cells from amyloidotic mice (amyloidotic peritoneal cells) are capable of processing the precursor protein of secondary amyloidosis, serum amyloid A (SAA) to amyloid fibrils, the peritoneum is a rare site for amyloid deposition. This is considered to be due to a deficiency of SAA in the peritoneum. To increase the supply of SAA to the peritoneum, ascitic fluid containing about the same protein constituents as in the serum was induced in mice. Amyloidotic peritoneal cells were packed in a microchamber which was shielded with filter membranes, and cultured in ascitic fluid supplemented with additional inflammatory factors. On the 7th day, Congo red-positive structures which showed green birefringence under polarized light were found inside and occasionally outside the chamber. By anti-AA or -SAA immunostaining, amyloid deposits and the cell surfaces of macrophages were positive. Immunologic depletion of T- and B-lymphocytes from the amyloidotic peritoneal cells did not adversely effect the amyloid formation in microchambers. These results suggest that either ascitic fluid containing sufficient amounts of SAA, or peritoneal macrophages with a high amyloid enhancing factor (AEF) activity are indispensable for AA amyloid fibrillogenesis in the peritoneum.

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