EVALUATION OF CD MARKERS IN LYMPHOMA STEM CELLS BY FLOW CYTOMETRY

Most cancer were diagnosed by using a few simple stains and looking at the appearance of cells under the microscope. But there were only a few types of lymphoma cancer that could easily be distinguished in this way, and it often appeared that tumors of the same cell type behaved very differently in different individuals. It was obvious that something else was going on at the molecular level to account for these differences. As medical diagnosis progressed to the study of molecules, new techniques such as flow cytometry were devised to identify specific molecules, located on the surface of white blood cells (CD markers). In this study was carried out to assess the relation between lymphoma stem cells and 3 from specific CD markers which are CD 19, CD23 and CD 24by using flowcytometry technique to help diagnose lymphoma cancer. Conclusively, in this study, CD 24 marker showed high positivity in Hodgkin lymphoma disease, and can be used as a diagnostic marker in this type of haematological disease and also help in its treatment. In addition, it can help in diagnosis of Non-Hodgkin lymphoma patients. This CD19 & CD23 can help in the diagnosis of Hodgkin lymphoma patients as these two markers showed also positivity in these cases.