从植物性食品中产生超氧化物的耐热复合物:分离、纯化和性能

R. Simonyan, G. Simonyan, A. S. Alexanyan, M. A. Babayan, S. Alexanyan, M. Simonyan
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摘要

源自亚美尼亚植物性食物——番茄(Solanum lycopersicum esculentum),胡萝卜(Daucus carota subsp.)。本文首次分离纯化了含NADPH蛋白组分(NPC)与铁(III) - NPC-铁(III)之间的超氧化物(О2)复合物。同时,在pH为9,5和4,8的条件下对这些配合物进行了分馏[1]。NPC-Nox的同工型是高热稳定性的生物系统(高达100℃)。这些配合物产生О2的直接机制是,由于电子从NPC转移到Fe(III),然后转移到О2,使其还原到О2。作为npc的底物,fe (III)不是游离的NADPH,而是与NADPH相连的蛋白组分(PC)。这些配合物在可见光区的光吸收光谱形式基本不同,但在紫外区观察到蛋白质在260 ~ 280 nm处的最大光吸收特征。从番茄中提取的复合物比含量(mg/g)高于胡萝卜、绿豆和马铃薯。研究了NPC组成中NADPH的含量与合成的О2在均相(溶液)和气相中的固定浓度成正比关系。从上述复合物中分离的NPC,以NADPH为代价,仅显示还原(抗氧化)作用,并在体外激活NADPH氧化酶(Nox),特别是从红细胞膜(ЕМ)和白细胞膜(LM)。通过将这些配合物的微乳白色水溶液与氧气吹制,产生气相О2 +,气相通过硅管或玻璃管传递氧气。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Superoxide-producing thermostable complex from plant foods: isolation, purification and properties
From Armenian plant food – tomato (Solanum lycopersicum esculentum), carrot (Daucus carota subsp. sativus), green bean (Phaseolus vulgaris) and potato (Solanum tuberosum), the isoforms of superoxide (О2) producing complexes between NADPH containing protein component (NPC) and Fe(III) – NPC-Fe(III), were isolated and purified for the first time. At the same time, the fractionation of these complexes at pH 9,5 and 4,8 were carried out [1]. The isoforms of NPC-Nox are high thermostable biosystems (up to 100oC). The immediately mechanism of the production of О2 by these complexes, due to the transfer of the electron from NPC to the Fe(III), then to О2 for its reduction up to О2 was conditioned. As a substrate for NPC-Fe(III) is not free NADPH, but NADPH is connected with the protein component (PC). The forms of optical absorption spectra of these complexes in visible region essentially were differed, although, in UV region the characteristic for the proteins maximal optical absorption at 260-280 nm were observed. The higher specific content (mg/g) for complexes from tomato, than from carrot, green bean and potato were observed. The direct proportional dependence between the content of the NADPH in the composition of NPC and stationary concentration of produced О2 in homogenous phase (in solution) and gas phase were presented. The isolated NPC from indicated above complexes, at the expense of NADPH, only the reductive (antioxidant) effect was indicated and was activated the NADPH oxidase (Nox) in vitro, in particular from erythrocytes membranes (ЕМ) аnd leukocytes membranes (LM). By the blowing of the slightly opalescent aqueous solutions of these complexes with oxygen the gas phase О2 were produced, which were transferred oxygen through the silicone or glass tubes.
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