肝蛋白酪氨酸磷酸酶的异质性。

P A Gruppuso, J M Boylan
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引用次数: 0

摘要

我们在大鼠肝脏的三个亚细胞区室中发现了蛋白酪氨酸磷酸酶家族的多个成员;膜、细胞骨架和细胞溶胶。基于底物特异性、大小和与人胎盘PTP1B抗肽抗血清的反应性的表征表明,Triton X-100颗粒膜提取物中至少存在三种ptpase。其中,600 kDa中的一个具有跨膜、受体样酶的特征。第四个颗粒PTPase (70 kDa)代表一个独特的细胞骨架PTPase。细胞质溶胶含有一个主要的PTPase物种,在Western免疫印迹中被检测为41 kDa蛋白。这些数据表明存在多种肝脏PTPases,其结构和亚细胞定位的差异可能反映了功能的异质性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Heterogeneity of hepatic protein tyrosine phosphatases.

We have identified multiple members of the protein tyrosine phosphatase family in three subcellular compartments from rat liver; membrane, cytoskeleton and cytosol. Characterization based on substrate specificity, size, and reactivity with an anti-peptide antiserum against human placental PTP1B indicate the presence of at least three PTPases in Triton X-100 extracts of particulate membranes. Of these, one of 600 kDa possesses characteristics of a transmembrane, receptor-like enzyme. A fourth particulate PTPase (70 kDa) represents a distinct cytoskeletal PTPase. Cytosol contains one main PTPase species which was detected as a 41 kDa protein in Western immunoblots. These data indicate the existence of multiple hepatic PTPases whose differences in structure and subcellular localization may reflect functional heterogeneity.

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