Immune status of broiler chickens experimentally infected with avian E.coli supplemented with amino acid mixture rich in Glycine

Meat producing broiler chickens may needs dietary amino acids of animal origin to obtain high growth, good health, muscles yield and healthy immune response. To investigate the effects of the amino acids mixture from animal origins rich in glycine on performance and immunity of broiler chickens an in vivo experiment was conducted at which T1 andT2 groups supplemented with amino acid mixture in every 10 days for successive 3 days, 8 hr. daily interval at dose 1 ml/L in drinking water and the second ( T2 group) was experimentally challenged with avian pathogenic E.coli (APEC) and the third group (T3) had no supplementation. The obtained results showed that amino acids mixture treated groups( T1 and T2) had  a remarkable immune-modulation as noticed by  significant  elevation of phagocytic count and activities(lysozymes and nitric oxide production) ,expression of  IL-4, IL-10 and  interferon- gamma-genes using RT-PCR. Performance studies proved that there was an improvement of body weight gain and  lowered mortality rate in amino acids treated group.