乳酸菌基因工程与培养的方向与方法供初学者参考

Japanese Journal of Lactic Acid Bacteria Pub Date : 2019-03-14 DOI:10.4109/jslab.30.8

K. Okano

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引用次数: 0

摘要

乳酸菌的基因工程对初学者来说是一项艰巨的任务，因为感兴趣的菌株差异很大，遗传工具的获取也很困难。本文根据作者的经验，对宿主菌株和载体的选择、实验方向和方法进行了综述;乳酸菌转化;表达体系优化;基因中断/插入;重组体的培养

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The directions and methods of genetic engineering and cultivation of lactic acid bacteria for the beginners

Genetic engineering of lactic acid bacteria is a difficult task for the beginners due to large difference in strains of interest and poor access for genetic tools. In this review, based on the author’ s experiences, experimental directions and methods for selecting the host strain and vectors; transformation of lactic acid bacteria; optimization of expression system; gene disruption/insertion; cultivation of recombinants

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Japanese Journal of Lactic Acid Bacteria

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