肾移植术后早期尿内肽酶24.11的病理释放。

Enzyme Pub Date : 1992-01-01 DOI:10.1159/000468808
J Nortier, D Abramowicz, P Kinnaert, M Deschodt-Lanckman, J L Vanherweghem
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引用次数: 6

摘要

采用合成肽底物,用荧光光谱法测定了人尿中的内多肽酶24.11 (EC 3.4.24.11)酶活性。测定10例肾移植术后2周内24小时尿内肽酶24.11输出量(Uendo)。在9例患者中,Uendo水平在术后第1天和/或第2天大幅增加(Uendo峰值的平均+/- SEM为624 +/- 122微克/24小时,p = 0.0003,而健康对照人群的峰值为239 +/- 20微克/24小时)。无论患者接受OKT3 (n = 6)还是环孢素A (n = 3)作为原发性免疫抑制,都发生了这种情况。术后第3天至第5天,Uendo恢复正常。我们得出结论,肾移植与尿中内肽酶24.11的早期和显著释放有关。这可能是由于缺血和/或免疫抑制药物对近端小管上皮的潜在毒性作用。尿内肽酶24.11作为尿小管损伤标志物的临床应用价值仍有待评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Pathological release of urinary endopeptidase 24.11 early after renal transplantation.

Endopeptidase 24.11 (EC 3.4.24.11) enzymatic activity was spectrofluorimetrically measured in human urine, using a synthetic peptidic substrate. Urinary endopeptidase 24.11 output (Uendo) was determined in 24-hour urine samples of 10 kidney transplant recipients during the first 2 weeks after surgery. In 9 patients, a large increase in Uendo levels was noted during the 1st and/or the 2nd postoperative days (mean +/- SEM of peak Uendo 624 +/- 122 micrograms/24 h, p = 0.0003 as compared to 239 +/- 20 micrograms/24 h in a healthy control population). This occurred whether patients received OKT3 (n = 6) or cyclosporine A (n = 3) as primary immunosuppression. Uendo returned to normal between the 3rd and the 5th postoperative day. We conclude that renal transplantation is associated with an early and marked release of endopeptidase 24.11 in urine. This could be due to the potentially toxic effects of ischemia and/or immunosuppressive drugs on the proximal tubular epithelium. The clinical usefulness of urinary endopeptidase 24.11 as a marker of tubular injury remains to be assessed.

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