常规PCR与培养法检测呼吸道疾病患者常见真菌病原体的比较

H. Rashied, A. Al-Attraqchi, Amar Kasim Muhmmed
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摘要

本研究旨在通过常规方法和分子方法评估呼吸道疾病患者侵袭性真菌感染的频率。本研究包括从2019年9月至2020年4月期间在巴格达医学教学医院和AL-Emamain AL-Khadhymian医疗城就诊的年龄在(20-80)岁之间的呼吸系统疾病患者(男性79名,女性38名)收集的117份支气管肺泡灌洗(BAL)样本。本研究PCR法与培养法对比结果显示,117份真菌感染样品中,培养法检出30份(25.6%),PCR法检出24份(20.5%),其中最常见的病原真菌是念珠菌,其次是曲霉,以培养法作为对照PCR技术的金标准,结果显示PCR的敏感性为86.6%,特异性为100%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Conventional PCR versus Culture Method to Detect Common Fungal Pathogens in Patients with Respiratory Diseases
The study aimed to assess the frequency of invasive fungal infection in patients with respiratory diseases by conventional and molecular methods. This study included 117 Broncho alveolar lavage (BAL) samples were collected from patients with respiratory disease (79 male and 38 female) with ages ranged between (20-80) years, who attended Medicine Baghdad Teaching hospital and AL-Emamain AL-Khadhymian Medical City, during the period from September 2019 to April 2020. The results in PCR versus culture methods in this study showed that out of 117 samples of fungal infections 30(25.6 %) were detected by culture method, while the 24(20.5%) samples were detected by PCR technique, the most commonly diagnosed pathogenic fungi is Candida spp. followed by Aspergillus spp. By considering the culture method as a gold standard against the PCR technique, the results show that the sensitivity and specificity of PCR were (86.6%) and (100%) respectively.
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