兔肝锌金属硫蛋白的克级纯化及制备。

Preparative biochemistry Pub Date : 1992-06-01 DOI:10.1080/10826069208021365

R D Comeau, K W McDonald, G L Tolman, M Vasak, F A Liberatore

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引用次数: 26

摘要

提出了一种用凝胶过滤和阴离子交换色谱法纯化兔肝金属硫蛋白(MT)克量的简化方法。MT的纯化采用阴离子交换间歇洗脱色谱法，大大缩短了过程。讨论了用于粗MT和纯化MT的定量技术。本文还介绍了以Cd、ZnMT为原料制备大量ZnMT的方法。

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Gram scale purification and preparation of rabbit liver zinc metallothionein.

A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd,ZnMT.

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Preparative biochemistry

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