木薯M1V4突变体诱导群体的多样性

M. Kinyua, H. Okwaro
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引用次数: 0

摘要

摘要木薯基因组较小(约770 Mb),被认为是一种具有高杂合性的异源多倍体,严重阻碍了木薯有用基因渗入的速度和方便性。突变技术形成了木薯等作物变异开发的经典程序,而遗传标记可以用来揭示种质中的遗传变异。本研究旨在通过表型和遗传标记程序确定诱变木薯早期种群的遗传相关性。通过表型和遗传性状的检测,在温室育苗期和田间成虫期对M1V4代木薯诱变群体的多样性进行了检测。所选突变体的株高、叶上裂片数、叶柄颜色、每茎节数和PCR产物凝胶分离度与亲本相比存在显著差异。例如,10个突变体株高为16 ~ 113cm,亲本株高为24.33 cm;节数为11 ~ 24个,亲本株高13个。突变系的多样性已得到证实,可以进行进一步的研究,以量化木薯育种和生产的变异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diversity of Induced M1V4 Mutant Population of Cassava (Mahinot Esculanta Crantz)
ABSTRACT Cassava owns a small genome (about 770 Mb), it is considered as an allopolyploid with a high level of heterozygosity characteristics which severely hamper the speed and ease of introgressing useful genes in cassava. Mutation techniques form a classical procedure of development of variation in crops like cassava while genetic markers can be used to reveal genetic variation in the germplasm. This study was undertaken to determine the genetic relatedness of mutagenised cassava early generation populations through phenotypic and genetic marker procedures. Diversity of mutagenised population of cassava at M1V4 generation was examined at seedling stage in the greenhouse and at adult stage in the field through checking phenotypic and genetic traits. Plant height, number of lobes on leaves, petiole colour, number of nodes per stem and gel separation of PCR products developed from SSR markers differed significantly between selected putative mutants as well as in comparison with parent line. For example plant height of the 10 selected mutants ranged from 16 cm to 113cm where the parent grew to 24.33 cm while the number of nodes ranged from 11 to 24 with the parent having 13 nodes. Diversity of the mutant lines was demonstrated and further studies can be undertaken to quantify the variation for breeding and production of cassava.      
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