细胞色素C氧化酶和多阴离子对铁细胞色素C碱性转变的影响。

Biochemistry international Pub Date : 1992-12-01

M Antalík, M Bona, J Bágelová

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引用次数: 0

摘要

应用肝素、聚腺苷酸、聚谷氨酸和聚半胱甘酸后，细胞色素c的电子吸收谱发生了类似于在中性ph下应用细胞色素c氧化酶后的变化。细胞色素c与多阴离子形成的复合物保留了Met-80与血红素铁的结合。细胞色素c氧化酶和多阴离子使细胞色素c碱性转化的表观pKa提高了一个数量级。

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Effect of cytochrome C oxidase and polyanions on the alkaline transition of ferricytochrome C.

Application of heparin, polyadenylate, polyglutamate and polygalacturonate resulted in changes in the electron absorption spectrum of cytochrome c that resembled those after cytochrome c oxidase application at neutral pH. The formed complexes of cytochrome c with polyanions retain the bond of Met-80 with heme iron. Cytochrome c oxidase and the polyanions increased the apparent pKa of alkaline transition of cytochrome c by an order of magnitude.

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Biochemistry international

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