前列腺素E2和环AMP对培养巨噬细胞摄取免疫球蛋白G复合物的影响。

Eicosanoids Pub Date : 1992-01-01

J Mattana, P C Singhal

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引用次数: 0

摘要

巨噬细胞(MP)对免疫复合物的摄取在循环免疫复合物增加的疾病状态中可能是重要的。我们进行了本研究，以确定前列腺素E2 (PGE2)和腺苷3'，5'-环单磷酸腺苷(cAMP)对MP摄取免疫球蛋白G复合物(IgG复合物)的影响。采用PGE2、磷酸二酯酶抑制剂3-异丁基-1-甲基黄嘌呤(IBMX)、PGE2 + IBMX、环氧合酶抑制剂吲哚美辛(IM)和3′，5′-环单磷酸二丁基腺苷(DBcAMP)预处理后，在建立的MP细胞系J774.16中测定了125i - igg -金颗粒(IgG-gold)的摄取。浓度从(10(-12))到(10(-5)M的PGE2预孵育显示(10(-6))和(10(-5)M)(每孔每分钟计数，PGE2 [10(-6) M]， 5,401 +/- 140)显著减少igg -金的摄取;对照组17,150 +/- 493,p < 0.001);(pge2 [10(-5) m]， 3,835 +/- 172;对照组17,150 +/- 493,p < 0.001)。单独IBMX (10(-3) M)没有显著改变igg -金摄取(IBMX, 14,450 +/- 1938;控制，14,840 +/- 995,p小于1.0)。PGE2 (10(-6) M) + IBMX (10(-3) M)显著抑制igg -金摄取(每孔每分钟计数，PGE2 + IBMX, 3,659 +/- 129;对照组18296 +/- 486,p < 0.001)。与对照组相比，单独使用PGE2 (10(-6) M)也抑制了igg -金的摄取(PGE2, 4,578 +/- 105;对照组，18296±486,p < 0.001)。(摘要删节250字)

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Effects of prostaglandin E2 and cyclic AMP on uptake of immunoglobulin G complexes by cultured macrophages.

The uptake of immune complexes by macrophages (MP) may be important in disease states in which circulating immune complexes are increased. We undertook the present study to determine the effects of prostaglandin E2 (PGE2) and adenosine 3',5'-cyclic monophosphate (cAMP) on the uptake of immunoglobulin G complexes (IgG complexes) by MP. The uptake of 125I-IgG-gold particles (IgG-gold) was measured in the established MP cell line J774.16 following pretreatment with PGE2, the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX), PGE2 plus IBMX, the cyclooxygenase inhibitor indomethacin (IM), and dibutyryladenosine 3',5'-cyclic monophosphate (DBcAMP). Preincubation with PGE2 at concentrations from (10(-12)) to (10(-5) M) revealed significantly diminished uptake of IgG-gold at (10(-6)) and (10(-5) M) (in counts per min per well, PGE2 [10(-6) M], 5,401 +/- 140; control, 17,150 +/- 493, p less than 0.001); (PGE2 [10(-5) M], 3,835 +/- 172; control, 17,150 +/- 493, p less than 0.001). IBMX (10(-3) M) alone did not significantly alter IgG-gold uptake (IBMX, 14,450 +/- 1938; control, 14,840 +/- 995, p less than 1.0). PGE2 (10(-6) M) plus IBMX (10(-3) M) significantly suppressed IgG-gold uptake (in counts per min per well, PGE2 plus IBMX, 3,659 +/- 129; control 18,296 +/- 486, p less than 0.001). PGE2 (10(-6) M) alone also suppressed IgG-gold uptake versus control (PGE2, 4,578 +/- 105; control, 18,296 +/- 486, p less than 0.001).(ABSTRACT TRUNCATED AT 250 WORDS)

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Eicosanoids

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