糖尿病对大鼠Guerin癌及局灶周围组织胰岛素样生长因子及其载体蛋白水平的影响

E. Frantsiyants, V. Bandovkina, I. Kaplieva, E. Surikova, I. Neskubina, Y. Pogorelova, N. Cheryarina, P. Kachesova, L. Nemashkalova
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引用次数: 1

摘要

研究目的:糖尿病(DM)被认为是癌症发病率和死亡率较高的独立危险因素。胰岛素样生长因子及其载体蛋白(IGF和IGFBP)系统与高血糖为癌细胞的增殖和转移创造了有利条件。材料和方法。将近交系雄性和雌性大鼠分为两组,每组8只:对照组-患有Guerin癌;实验动物通过单次腹腔注射四氧嘧啶(150 mg/kg体重)复制DM。肿瘤生长10 d后,采用elisa法测定肿瘤及病灶周围IGF和IGFBP水平。女性DM使肿瘤组织和局周组织的葡萄糖水平分别升高1.8倍和8.1倍(p < 0.05),而引起IGF-I的变化相反,肿瘤组织升高6.3倍,局周组织降低3.2倍;因此,具有小原发淋巴结的肿瘤更具有“侵袭性”,更容易转移。在男性中,诱导DM使癌组织中葡萄糖、IGF-II和IGFBP2水平分别下调8.4倍、3.1倍和1.7倍(p < 0.05),而在葡萄糖水平不变的情况下,IGF-I和IGFBP2水平分别升高1.4倍和1.3倍(p < 0.05);结果肿瘤体积超过标准生长值,未发生内脏器官转移。我们发现,在患有糖尿病的Guerin癌的大鼠中,肿瘤及其局点周围组织中葡萄糖和IGF水平的变化存在性别差异;这些差异可能决定了雄性和雌性大鼠不同的肿瘤生长动态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of diabetes mellitus on levels of insulin-like growth factors and their carrier proteins in Guerin’s carcinoma and it’s perifocal tissue in rats
Purpose of the study. Diabetes mellitus (DM) is considered an independent risk factor for higher cancer incidence and death rates. The system of insulin-like growth factors and their carrier proteins (IGF and IGFBP) and hyperglycemia create favorable conditions for the proliferation and metastasis of cancer cells.Materials and methods. Outbred male and female rats were divided into groups (n = 8 each): controls - with Guerin's carcinoma; main group - Guerin's carcinoma growing in presence of DM. Experimental DM was reproduces in animals by the single intraperitoneal alloxan injection (150 mg/kg body weight). After 10 days of the carcinoma growth, levels of IGF and IGFBP in the tumor and in it's perifocal area were measured using ELISA.Results. DM in females upregulated levels of glucose both in the tumor and in perifocal tissues by 1.8 (p < 0.05) and 8.1 times, respectively, but caused opposite changes in IGF-I - it's increase by 6.3 times in the tumor and decrease by 3.2 times in the perifocal area; as a result, such tumors with small primary nodes were more "aggressive" and actively metastasized. In males, induced DM downregulated levels of glucose, IGF-II and IGFBP2 in the carcinoma by 8.4, 3.1 and 1.7 (p < 0.05) times, respectively, and increased levels of IGF-I and IGFBP2 by 1.4 and 1.3 times (p < 0.05) in the perifocal area without changing glucose levels; as a result, tumor volumes exceeded the values in the standard growth, without metastasizing into visceral organs.Conclusion. We revealed gender differences in changing levels of glucose and IGF both in the tumor and in it's perifocal tissue in rats with Guerin's carcinoma growing in presence of DM; these differences could determine different tumor growth dynamics in male and female rats.
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