2536: Ex vivo hypothermic perfusion of amputated porcine forelimbs promotes reduced molecular damage to skeletal muscle when compared with conventional cold storage

2536: Ex vivo hypothermic perfusion of amputated porcine forelimbs promotes reduced molecular damage to skeletal muscle when compared with conventional cold storage Nicco Krezdorn, Dharaniya Sakthivel, Mario A. Aycart, MD, Muayyad Alfhefzi, Thet-Su Win, Harriet Kiwanuka, Ericka Bueno, Indranil Sinha, and Bohdan Pomahac Brigham and Women’s Hospital, Boston, MA, USA Background Limb allotransplantation is largely limited by the allowable ischemia time in limbs Muscle makes up most of the volume of isolated limbs, and its maximum allowable ischemia time is currently in the order of 4–6–h Extracorporeal perfusion devices may support safe extension of the allowable ischemia time for limb tissues The aim of this study was to assess the effect of different storage modalities on gene expression in porcine forelimbs. Methods Porcine forelimbs were amputated and either perfused with oxygenated acellular supplemented perfusion solution (Perfadex ) for 12 h at 10 C (treatment; n D 3) or stored on ice slurry at 4 C (control; n D 2) before replantation Gene expression profiles in muscle tissues from the amputated limb were analyzed after 2 h on ice as well as after 2–h and 12–h perfusion using a hypoxia gene expression qPCR array of 84