噻吩酮化合物对嗜水气单胞菌运动和酪酶产生的影响

Ayu Ashari Margareth Sinaga, Pande Gde Sasmita Julyantotro, D. Pebriani
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引用次数: 0

摘要

运动和酪蛋白酶的产生是细菌毒力因子的一部分,由细菌细胞间通讯系统调节,通常称为群体感应(QS)。本研究旨在确定QS抑制剂复方噻吩酮对这些毒力因子的降低作用。该研究于2019年12月至2020年3月在乌达亚纳大学海洋科学与渔业学院渔业科学实验室进行。本研究测试了3种不同的处理,每种处理重复3次。A组作为对照(不加噻吩酮),B组(加5 μ M噻吩酮),C组(加10 μ M噻吩酮)。结果表明,噻吩酮类化合物对嗜水气单胞菌的QS系统具有抑制作用。可以看出,与未添加噻吩酮类化合物相比,嗜水单胞菌的运动性和酪酶酶活性显著降低(P<0.05)。处理A在22 h产生的酪酶酶平均直径为21.40±0.36 mm,处理B为19.70±0.2 mm,处理C为17.87±0.05 mm。而在运动性方面,22小时处理A的平均直径为6.57±0.61 mm,处理B的平均直径为5.67±0.35 mm,处理C的平均直径为5.10±0.6 mm。这些结果表明,QS噻吩酮抑制剂化合物可以降低致病菌嗜水杆菌的毒力因子,即运动性和酪蛋白酶的产生。与治疗A和B相比,治疗C可以降低毒力因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Thiophenone Compound on Motility and Caseinase Production of Aeromonas hydrophila
Motility and caseinase production are part of bacterial virulence factors which are regulated by a bacterial intercellular communication system, often called quorum sensing (QS). This study aims to determine the effect of the QS inhibitor compound thiophenone in reducing those virulence factors. This research was conducted at the Laboratory of Fisheries Science, Faculty of Marine Science and Fisheries, Udayana University, from December 2019 to March 2020. This study tested 3 different treatments with 3 repetitions for each treatment. Treatment A as control (without the addition of thiophenone), treatment B (addition of 5 ?M thiophenone), and C treatment (addition of 10 ?M thiophenone). The results showed that thiophenone compounds can inhibit the QS system of Aeromonas hydrophila. It can be seen from the significantly reduced (P<0.05) motility and caseinase enzyme activity of A. hydrophila compared to without addition of thiophenone compounds. The average diameter of the caseinase enzyme production produced in treatment A at 22 hours was 21.40±0.36 mm, in treatment B was 19.70±0.2 mm and in treatment, C was 17.87±0.05 mm. Whereas in motility, the resulting average diameter in treatment A at 22 hours was 6.57±0.61 mm, in treatment B was 5.67±0.35 mm and in treatment, C was 5.10±0.6 mm. These results indicate that the QS thiophenone inhibitor compound can reduce virulence factors, namely motility and caseinase production from pathogenic bacteria A. hydrophila. Treatment C can decrease virulence factors compared to treatment A and B.
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