Application of ABO Blood Grouping Monoclonal Antibodies to Forensic Samples

Sensitivity and specificity of commercially available ABO blood grouping monoclonal antibodies for medical use were examined for applying them to forensic blood typing. We have applied some monoclonal antibodies to hemagglutination test, enzyme-linked immunosorbent assay (ELISA) and absorption-elution test.   Hemagglutination titer of these antibodies distributed ranging from ×4 to ×512.   All antibodies examined could be used for detecting ABO blood type from secretor's saliva by ELISA method and using five anti-A antibodies blood group substances could be detected from non-secretor's saliva. Using anti-A and anti-B antibodies blood type could be detected from secretor's semen but using any antibody blood type could not be detected from non-secretor's semen. We could not get a correlation between hemagglutination titer and sensitivity on ELISA detected from saliva and semen.   At absorption-elution test, using most of the anti-A and anti-B antibodies blood type could be detected of bloodstains and secretor's body fluid stains. Using several antibodies blood type could not be detected of non-secretor's samples. Using all of anti-A antibodies and some of anti-B antibodies it was failed to detect blood type of hair samples. Using all of the anti-H antibodies blood group substances could not be detected of stain or hair samples.   This result suggested that we have to select suitable monoclonal antibodies of ABO blood grouping for each evidential sample and each blood typing method.